ProtParam
User-provided sequence:
10 20 30 40 50 60
MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS ELEKGLSKKG
70 80 90 100 110 120
GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG GDRTFDTTQS KYRLPDAMRT
130 140 150 160 170 180
TQNPDELWEF IADSLKAFID EQFPQGISEP IPLGFTFSFP ASQNKINEGI LQRWTKGFDI
190 200 210 220 230 240
PNIENHDVVP MLQKQITKRN IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY
250 260 270 280 290 300
YDVCSDIEKL QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT
310 320 330 340 350 360
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI EEDPFENLED
370 380 390 400 410 420
TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI AAICQKRGYK TGHIAADGSV
430 440 450 460 470 480
YNRYPGFKEK AANALKDIYG WTQTSLDDYP IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS
VGIIGA
References and
documentation are available.
Number of amino acids: 486
Molecular weight: 53942.47
Theoretical pI: 5.16
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2416
Hydrogen H 3787
Nitrogen N 633
Oxygen O 731
Sulfur S 17
Formula: C
2416H
3787N
633O
731S
17
Total number of atoms: 7584
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46090
Abs 0.1% (=1 g/l) 0.854, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 0.850, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.75
This classifies the protein as stable.
Aliphatic index: 82.10
Grand average of hydropathicity (GRAVY): -0.328