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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS ELEKGLSKKG

70 80 90 100 110 120
GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG GDRTFDTTQS KYRLPDAMRT

130 140 150 160 170 180
TQNPDELWEF IADSLKAFID EQFPQGISEP IPLGFTFSFP ASQNKINEGI LQRWTKGFDI

190 200 210 220 230 240
PNIENHDVVP MLQKQITKRN IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY

250 260 270 280 290 300
YDVCSDIEKL QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT

310 320 330 340 350 360
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI EEDPFENLED

370 380 390 400 410 420
TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI AAICQKRGYK TGHIAADGSV

430 440 450 460 470 480
YNRYPGFKEK AANALKDIYG WTQTSLDDYP IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS


VGIIGA


References and documentation are available.

Number of amino acids: 486 Molecular weight: 53942.47 Theoretical pI: 5.16
Amino acid composition: 
Ala (A) 34 7.0% Arg (R) 19 3.9% Asn (N) 19 3.9% Asp (D) 34 7.0% Cys (C) 4 0.8% Gln (Q) 22 4.5% Glu (E) 32 6.6% Gly (G) 41 8.4% His (H) 5 1.0% Ile (I) 40 8.2% Leu (L) 35 7.2% Lys (K) 35 7.2% Met (M) 13 2.7% Phe (F) 24 4.9% Pro (P) 29 6.0% Ser (S) 25 5.1% Thr (T) 30 6.2% Trp (W) 4 0.8% Tyr (Y) 16 3.3% Val (V) 25 5.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 66 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2416 Hydrogen H 3787 Nitrogen N 633 Oxygen O 731 Sulfur S 17 Formula: C2416H3787N633O731S17 Total number of atoms: 7584 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 46090 Abs 0.1% (=1 g/l) 0.854, assuming all pairs of Cys residues form cystines Ext. coefficient 45840 Abs 0.1% (=1 g/l) 0.850, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 39.75 This classifies the protein as stable. Aliphatic index: 82.10 Grand average of hydropathicity (GRAVY): -0.328