ProtParam
User-provided sequence:
10 20 30 40 50 60
MVIILLGLCT LGFPRTAFCP SIMTNSTVSI NTPPPYLTLA CNEKLPTVMS IAGSDSSGGA
70 80 90 100 110 120
GVEADIKTIT AHRCYAMTCV TTLTAQTPVK VYGAHNIPKK MVSQILDANL QDMKCNVIKT
130 140 150 160 170 180
GMLTVDAIEV LHEKLLQLGE NRPKLVIDPV LCAASDSSPT GKDVVSLIIE KISPFADILT
190 200 210 220 230 240
PNISDCFMLL GENREVSKLQ DVLEIAKDLS RITNCSNILV KGGHIPCDDG KEKHITDVLY
250 260 270 280 290 300
LGAEQKFITF KGQFVNTTRT HGAGCTLASA IASNLARGYS LSQSVYGGIE YVQNAIAIGC
310 320 330 340 350 360
DVTKKAVKVG PINHVYAVEI PLEKMLTDEC FTASDAVPKK PIEGSLDKIP GGSFFNYLIN
370 380 390 400 410 420
HPKVKPHWDA YVNHEFVKRV ADGTLERKKF QFFIEQDYLY LIDYVRVCCV TGSKSPTLED
430 440 450 460 470 480
LEKDLVIADC ARNELNEHER RLREEFGVKD PDYLQKIKRG PALRAYCRYL IDISRRGNWQ
490 500 510 520 530 540
EIVVALNPCL MGYVYAVDKV KDKITAAEGS IYSEWCDTCA SSFCYQAVLE GERLMNHILE
550 560 570
TYPPDQLDSL VTIFARGCEL ETNFWTAAME YE
References and
documentation are available.
Number of amino acids: 572
Molecular weight: 63307.04
Theoretical pI: 5.66
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2817
Hydrogen H 4478
Nitrogen N 740
Oxygen O 844
Sulfur S 34
Formula: C
2817H
4478N
740O
844S
34
Total number of atoms: 8913
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56155
Abs 0.1% (=1 g/l) 0.887, assuming all pairs of Cys residues form cystines
Ext. coefficient 54780
Abs 0.1% (=1 g/l) 0.865, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.77
This classifies the protein as stable.
Aliphatic index: 95.10
Grand average of hydropathicity (GRAVY): -0.036