ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVITDTAAWV QSQSRSIVDF ISAPNSNSSL PPYVQIGAAA AVTMGLGYYL SKSSSSTSGS 

        70         80         90        100        110        120 
YSFSGVTYSG NVKPLVDPMN QSRILPDGSR ISAYLKDDEL QAFLFEDART LYQGVRRGAR 

       130        140        150        160        170        180 
LSNNGPMLGR RVKQSDGSIP YVWESYNTIL ERADNVSVAF RELGVPTGNA ENIGIYSKNR 

       190        200        210        220        230        240 
AEWIITEFAT YNYSNVIVPI YETLGSEASI FILNQAEIKI VVCDDISKAT GLLKFKEQCP 

       250        260        270        280        290        300 
SLSTLVVMEP VTDELKTTAS SLGVEVLTFE DLEKIGKNAK TRPAHIPPTP EDLATICYTS 

       310        320        330        340        350        360 
GTTGTPKGVM LTHANVIADG VCMDFFKHSG IAATDSMISF LPLAHMLERV IESVCFCVGA 

       370        380        390        400        410        420 
KVGFYRGDIR VLAEDIKELK PTVVPVVPRV LNRLYDKVMS EVNKSTLKKM LFDFAISYKA 

       430        440        450        460        470        480 
RDMANFNIRN DGFFDNLVFK KIREGFGGRV RLMITGSAPL STNVLTFVRA AMGCVVVEGY 

       490        500        510        520        530        540 
GQTECVAACT VSMEGDSLAG HVGMVIPSCQ IKLVDVPELN YYAKDQAGEV CVKGHIIFKG 

       550        560        570        580        590        600 
YYKNEQQTKE TIDEDGWLHT GDIGRWTPEG TLKIVDRKKH IFKLSQGEYV APEKIENIYV 

       610        620        630        640        650        660 
RSKYVAQSFV HGESLKTCLI AIVVPDAEVL VPAMADQGIK GTFEELCNND AVKKAILDDM 

       670        680        690        700        710 
VAVGKKAGLF SFEQVRDISL SAEQFSVEND LLTPTLKSKR PKLKAHYSKQ LDAMYSKLP 

References and documentation are available.

Number of amino acids: 719

Molecular weight: 79015.78

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  54	  7.5%
Arg (R)  29	  4.0%
Asn (N)  29	  4.0%
Asp (D)  39	  5.4%
Cys (C)  13	  1.8%
Gln (Q)  20	  2.8%
Glu (E)  43	  6.0%
Gly (G)  51	  7.1%
His (H)  10	  1.4%
Ile (I)  47	  6.5%
Leu (L)  57	  7.9%
Lys (K)  49	  6.8%
Met (M)  19	  2.6%
Phe (F)  29	  4.0%
Pro (P)  31	  4.3%
Ser (S)  58	  8.1%
Thr (T)  42	  5.8%
Trp (W)   5	  0.7%
Tyr (Y)  27	  3.8%
Val (V)  67	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3528
Hydrogen    H	      5599
Nitrogen    N	       929
Oxygen      O	      1060
Sulfur      S	        32

Formula: C3528H5599N929O1060S32
Total number of atoms: 11148

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68480
Abs 0.1% (=1 g/l)   0.867, assuming all pairs of Cys residues form cystines


Ext. coefficient    67730
Abs 0.1% (=1 g/l)   0.857, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.19
This classifies the protein as stable.



Aliphatic index: 90.95

Grand average of hydropathicity (GRAVY): -0.057