ProtParam
User-provided sequence:
10 20 30 40 50 60
MVITSKPCVF LDRDGVIVVP EFRNGRTFAP KTLEGFSYFP DTKKTLVRLK TAGYLLIVVT
70 80 90 100 110 120
NQPDVGNGIT SLATIENMHN KMRQELPIDH IEVCYHSQKD NCHCRKPKAG MLLNSLKKFN
130 140 150 160 170 180
IIVQKSFMIG DRSSDIEAGR KLNLKTIFID HHYQEPITAN PDYICQDLSQ GVEIILREEN
A
References and
documentation are available.
Number of amino acids: 181
Molecular weight: 20563.80
Theoretical pI: 8.25
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 913
Hydrogen H 1464
Nitrogen N 252
Oxygen O 267
Sulfur S 10
Formula: C
913H
1464N
252O
267S
10
Total number of atoms: 2906
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7700
Abs 0.1% (=1 g/l) 0.374, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.362, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.89
This classifies the protein as unstable.
Aliphatic index: 92.04
Grand average of hydropathicity (GRAVY): -0.275