ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKALDRSLA LLKQEKIAVL YGGNSAEREV SLNSGQAIAK GLEDAGFNVV LIDTKVVPLT
70 80 90 100 110 120
DLVSNKIDRV FIALHGRGGE DGCLQGALEY LDIPYTGSNV LGSSLSMDKV RSKQIFKACG
130 140 150 160 170 180
IPTAPFTVVN KAEFSTLSLE NILADLGGRV MVKPANEGSS IGMAQAKTIA QLRNALIEAF
190 200 210 220 230 240
GFDTQVLLEA WIDGPEYTVT ILGDKALPAI HMETPREFYD YEAKYQSTST QYHCPCGLNE
250 260 270 280 290 300
RDENEIKSLS MKAFNATGAQ GWGRVDIMRN KTGEWQILEV NTVPGMTETS LVPKAAKVFG
310
LNFSELVTQI LQLSVKE
References and
documentation are available.
Number of amino acids: 317
Molecular weight: 34454.47
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1530
Hydrogen H 2453
Nitrogen N 407
Oxygen O 470
Sulfur S 12
Formula: C
1530H
2453N
407O
470S
12
Total number of atoms: 4872
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28670
Abs 0.1% (=1 g/l) 0.832, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.825, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.20
This classifies the protein as stable.
Aliphatic index: 95.99
Grand average of hydropathicity (GRAVY): -0.056