ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKETEYYDI LGIKPEATPT EIKKAYRRKA METHPDKHPD DPDAQAKFQA VGEAYQVLSD
70 80 90 100 110 120
PGLRSKYDQF GKEDAVPQQG FEDASEYFTA IFGGDGFKDW IGEFSLFKEL NEATEMFGKE
130 140 150 160 170 180
DEEGTAATET EKADESTDGG MVKHDTNKAE SLKKDKLSKE QREKLMEMEK KRREDMMKQV
190 200 210 220 230 240
DELAEKLNEK ISRYLIAVKS NNLEEFTRKL DQEIEDLKLE SFGLELLYLL ARVYKTKANN
250 260 270 280 290 300
FIMSKKTYGI SKIFTGTRDN ARSVKSAYNL LSTGLEAQKA MEKMSEVNTD ELDQYERAKF
310 320 330 340 350 360
ESTMAGKALG VMWAMSKFEL ERKLKDVCNK ILNDKKVPSK ERIAKAKAML FIAHKFASAR
370 380 390
RSPEEAEEAR VFEELILGEQ EKEHKKHTVA R
References and
documentation are available.
Number of amino acids: 391
Molecular weight: 44854.89
Theoretical pI: 5.63
Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 1975
Hydrogen H 3148
Nitrogen N 534
Oxygen O 623
Sulfur S 16
Formula: C
1975H
3148N
534O
623S
16
Total number of atoms: 6296
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.644, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.644, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.68
This classifies the protein as stable.
Aliphatic index: 66.21
Grand average of hydropathicity (GRAVY): -0.841