ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKGPGLYTE IGKKARDLLY KDHNSDQKFS ITTFSPAGVA ITSTGTKKGD LLLGDVAFQS
70 80 90 100 110 120
RRKNITTDLK VCTDSTFLIT ATVDEAAPGL RSIFSFKVPD QNSGKVELQY LHEYAGISTS
130 140 150 160 170 180
MGLTQNPTVN FSGVIGSNVL AVGTDVSFDT KSGNFTKINA GLSFTKEDLI ASLTVNDKGD
190 200 210 220 230 240
LLNASYYHIV NPLFNTAVGA EVSHKLSSKD STITVGTQHS LDPLTSVKAR VNSAGIASAL
250 260 270
IQHEWKPKSF FTISGEVDTK SIDKSAKVGL ALALKP
References and
documentation are available.
Number of amino acids: 276
Molecular weight: 29425.36
Theoretical pI: 8.77
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1311
Hydrogen H 2100
Nitrogen N 348
Oxygen O 412
Sulfur S 3
Formula: C
1311H
2100N
348O
412S
3
Total number of atoms: 4174
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.491, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 18.93
This classifies the protein as stable.
Aliphatic index: 89.02
Grand average of hydropathicity (GRAVY): -0.114