ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKIEVGSVG DSFSVSSLKA YLSEFIATLL FVFAGVGSAV AFAKLTSDGA LDPAGLVAIA 

        70         80         90        100        110        120 
IAHAFALFVG VSIAANISGG HLNPAVTLGL AIGGNITLIT GFFYWIAQCL GSIVACLLLV 

       130        140        150        160        170        180 
FVTNGKSVPT HGVSAGLGAV EGVVMEIVVT FALVYTVYAT AADPKKGSLG TIAPIAIGFI 

       190        200        210        220        230        240 
VGANILAAGP FSGGSMNPAR SFGPAVVSGD LSQIWIYWVG PLVGGALAGL IYGDVFIGSY 

       250 
EAVETREIRV 

References and documentation are available.

Number of amino acids: 250

Molecular weight: 25245.49

Theoretical pI: 5.21

Amino acid composition: 
Ala (A)  35	 14.0%
Arg (R)   3	  1.2%
Asn (N)   6	  2.4%
Asp (D)   6	  2.4%
Cys (C)   2	  0.8%
Gln (Q)   2	  0.8%
Glu (E)   7	  2.8%
Gly (G)  34	 13.6%
His (H)   3	  1.2%
Ile (I)  22	  8.8%
Leu (L)  24	  9.6%
Lys (K)   6	  2.4%
Met (M)   3	  1.2%
Phe (F)  15	  6.0%
Pro (P)   9	  3.6%
Ser (S)  20	  8.0%
Thr (T)  12	  4.8%
Trp (W)   3	  1.2%
Tyr (Y)   7	  2.8%
Val (V)  31	 12.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 9

Atomic composition:

Carbon      C	      1174
Hydrogen    H	      1836
Nitrogen    N	       282
Oxygen      O	       324
Sulfur      S	         5

Formula: C1174H1836N282O324S5
Total number of atoms: 3621

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27055
Abs 0.1% (=1 g/l)   1.072, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   1.067, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.36
This classifies the protein as stable.



Aliphatic index: 121.72

Grand average of hydropathicity (GRAVY): 1.008