ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKKVNNPLK IDYQNGIIEN RLLQIRNFKD VNTPKLINVW SIRIDPRDSK KVIELIRNDF 

        70         80         90        100        110        120 
QKNDPVSLRH LKRIRKDIET STLEVVLCSK EYICDEGEIN NKLKSIWVGT KKYELSDDIE 

       130        140        150        160        170        180 
VPEFAPSTKE LNNAWSVKYW PLIWNGNPND QILNDYKIDM QEVRNELSRA STLSVKMATA 

       190        200        210        220        230        240 
GKQFPMVSVF VDPSRKKDKV VAEDGRNCEN SLPIDHSVMV GIRAVGERLR EGVDEDANSY 

       250        260        270        280        290        300 
LCLDYDVYLT HEPCSMCSMA LIHSRVRRVV FLTEMQRTGS LKLTSGDGYC MNDNKQLNST 

       310        320 
YEAFQWIGEE YPVGQVDRDV CC 

References and documentation are available.

Number of amino acids: 322

Molecular weight: 37107.37

Theoretical pI: 5.95

Amino acid composition: 
Ala (A)  10	  3.1%
Arg (R)  20	  6.2%
Asn (N)  24	  7.5%
Asp (D)  25	  7.8%
Cys (C)   9	  2.8%
Gln (Q)  10	  3.1%
Glu (E)  23	  7.1%
Gly (G)  14	  4.3%
His (H)   4	  1.2%
Ile (I)  22	  6.8%
Leu (L)  26	  8.1%
Lys (K)  25	  7.8%
Met (M)   9	  2.8%
Phe (F)   7	  2.2%
Pro (P)  13	  4.0%
Ser (S)  23	  7.1%
Thr (T)  12	  3.7%
Trp (W)   6	  1.9%
Tyr (Y)  11	  3.4%
Val (V)  29	  9.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1628
Hydrogen    H	      2599
Nitrogen    N	       455
Oxygen      O	       499
Sulfur      S	        18

Formula: C1628H2599N455O499S18
Total number of atoms: 5199

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49890
Abs 0.1% (=1 g/l)   1.344, assuming all pairs of Cys residues form cystines


Ext. coefficient    49390
Abs 0.1% (=1 g/l)   1.331, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.80
This classifies the protein as stable.



Aliphatic index: 87.36

Grand average of hydropathicity (GRAVY): -0.507