ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKLTPELIN QSMQYINPCR ERELDLRGYK IPQIENLGAT LDQFDTIDLS DNDLRKLDNL
70 80 90 100 110 120
PHLPRLKCLL LNNNRILRIS EGLEEAVPNL GSIILTGNNL QELSDLEPLV GFTKLETICL
130 140 150 160 170 180
LINPVSTKPN YREYMAYKFP QLRLLDFRKI KQKDRQAAQE FFRTKQGKDV LKEISRKSKM
190 200 210 220 230 240
SAAAAIAAEA GNGKGRGSEG GRLANPQDMQ RIREAIKRAS SLAEVERLSQ ILQSGQLPDK
250 260
FQHEMEAVAQ NGAGHNGSGA VAMEY
References and
documentation are available.
Number of amino acids: 265
Molecular weight: 29744.11
Theoretical pI: 8.67
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1299
Hydrogen H 2130
Nitrogen N 380
Oxygen O 397
Sulfur S 10
Formula: C
1299H
2130N
380O
397S
10
Total number of atoms: 4216
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9065
Abs 0.1% (=1 g/l) 0.305, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.301, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.27
This classifies the protein as stable.
Aliphatic index: 93.21
Grand average of hydropathicity (GRAVY): -0.505