ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKMGEVWTN TGSALASLVF IYTIFERFFP YRLREHFEPL AQSLIGFIYP YIQITFHEYS 

        70         80         90        100        110        120 
GERFKRSDVY DAIQSYLSKD SSSRAKKLTA NTIKGNKSII LSMDDHEEIT DEFQGVKVWW 

       130        140        150        160        170        180 
QSKKHQSESR AISFYPKADE SRFYMLKFHR RDREVITKKY LNHVISEGKT IEVKNRERKL 

       190        200        210        220        230        240 
YSNNPSQNWS GYKQTKWSHV TFEHPATFDT LAMEYKKKEE IKNDLIKFSN SKDYYKKIGK 

       250        260        270        280        290        300 
AWKRGYLLFG PPGTGKSTMI AAMANLLEYD VYDLELTTVK DNTELRRLLI ETSGKSIIVI 

       310        320        330        340        350        360 
EDIDCSLDLT GQRKQKKDEE EDEDETSPIE KQMKKDQGEN KGSKVTLSGL LNFIDGLWSA 

       370        380        390        400        410        420 
CGGERIIVFT TNFIDKLDPA LIRKGRMDKH IEMSYCGFEA FKVLANNYLD AKEEDDNELF 

       430        440        450        460        470        480 
DEIKRLLEVE EIKMTPADVG ENLLKKSEVE TKEICLKRLI EALKEEKEEA KRRIEDEEKK 

       490        500        510 
KKEEEEIKRK KREEKKIKKE EKEEKEENET TMKD 

References and documentation are available.

Number of amino acids: 514

Molecular weight: 60347.68

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  22	  4.3%
Arg (R)  26	  5.1%
Asn (N)  20	  3.9%
Asp (D)  32	  6.2%
Cys (C)   4	  0.8%
Gln (Q)  12	  2.3%
Glu (E)  66	 12.8%
Gly (G)  24	  4.7%
His (H)   9	  1.8%
Ile (I)  38	  7.4%
Leu (L)  40	  7.8%
Lys (K)  68	 13.2%
Met (M)  12	  2.3%
Phe (F)  22	  4.3%
Pro (P)  11	  2.1%
Ser (S)  34	  6.6%
Thr (T)  28	  5.4%
Trp (W)   7	  1.4%
Tyr (Y)  20	  3.9%
Val (V)  19	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      2689
Hydrogen    H	      4261
Nitrogen    N	       717
Oxygen      O	       825
Sulfur      S	        16

Formula: C2689H4261N717O825S16
Total number of atoms: 8508

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68550
Abs 0.1% (=1 g/l)   1.136, assuming all pairs of Cys residues form cystines


Ext. coefficient    68300
Abs 0.1% (=1 g/l)   1.132, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.95
This classifies the protein as unstable.



Aliphatic index: 74.18

Grand average of hydropathicity (GRAVY): -0.847