ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE LRQNNGSCGI
70 80 90 100 110 120
VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT CDTKQEMLAL SKLTPSRQGN
130 140 150 160 170 180
PENDGSGAPF NAIIAVSFGK LIPGDLIRAV PLALNVHPSL LPRHKGSAPI QRALLEGDTY
190 200 210 220 230 240
TGVTIQTLHP DRFDHGAIVA QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG
250 260 270 280 290 300
ALGAQLLGQT LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL
310 320 330 340 350 360
GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD IKDCILVTCR
370 380 390 400
GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF L
References and
documentation are available.
Number of amino acids: 401
Molecular weight: 44616.72
Theoretical pI: 9.68
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1975
Hydrogen H 3199
Nitrogen N 575
Oxygen O 565
Sulfur S 18
Formula: C
1975H
3199N
575O
565S
18
Total number of atoms: 6332
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21930
Abs 0.1% (=1 g/l) 0.492, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.480, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.23
This classifies the protein as stable.
Aliphatic index: 91.52
Grand average of hydropathicity (GRAVY): -0.238