ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKNTVKHVD SSKHLEKVAS LIKEGKVKKI CVMVGAGIST AAGIPDFRSP ETGIYNNLQR 

        70         80         90        100        110        120 
FNLPYAEAVF DLSYFRKNPR PFYELAHELM PEKYRPTYTH YFIRLLHDKR LLQKCYTQNI 

       130        140        150        160        170        180 
DTLERLAGVP DKALIEAHGS FQYSRCIECY EMAETEYVRA CIMQKQVPKC NSCKGLIKPM 

       190        200        210        220        230        240 
IVFYGEGLPM RFFEHMEKDT KVCDMALVIG TSLLVHPFAD LPEIVPNKCQ RVLINREPAG 

       250        260        270        280        290        300 
DFGERKKDIM ILGDCDSQVR ALCKLLGWSD ELEKLIDTSV ETLTEEISLL SVDSTIEKNA 

       310        320        330 
SEQKKDDNSV NPFTKIEEKK KDEVTLLVSD DE 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 37915.83

Theoretical pI: 5.98

Amino acid composition: 
Ala (A)  17	  5.1%
Arg (R)  15	  4.5%
Asn (N)  12	  3.6%
Asp (D)  21	  6.3%
Cys (C)  11	  3.3%
Gln (Q)   9	  2.7%
Glu (E)  30	  9.0%
Gly (G)  15	  4.5%
His (H)   8	  2.4%
Ile (I)  21	  6.3%
Leu (L)  32	  9.6%
Lys (K)  31	  9.3%
Met (M)  10	  3.0%
Phe (F)  13	  3.9%
Pro (P)  16	  4.8%
Ser (S)  19	  5.7%
Thr (T)  16	  4.8%
Trp (W)   1	  0.3%
Tyr (Y)  12	  3.6%
Val (V)  23	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1685
Hydrogen    H	      2688
Nitrogen    N	       446
Oxygen      O	       503
Sulfur      S	        21

Formula: C1685H2688N446O503S21
Total number of atoms: 5343

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24005
Abs 0.1% (=1 g/l)   0.633, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.617, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.69
This classifies the protein as stable.



Aliphatic index: 87.47

Grand average of hydropathicity (GRAVY): -0.344