ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKRSKKSKS KRVTLKQKHK VLKKVKEHHK KKAKDAKKLG LHRKPRVEKD PGIPNDWPFK 

        70         80         90        100        110        120 
EQELKALEVR RARALEEIEQ KKEARKERAK KRKLGLVDDE DTKTEGETIE DLPKVVNVRD 

       130        140        150        160        170        180 
NSERAFYKEL VKVIELSDVI LEVLDARDPL GTRCTDMERM VMQAGPNKHL VLLLNKIDLV 

       190        200        210        220        230        240 
PREAAEKWLM YLREEFPAVA FKCSTQEQRS NLGWKSSKAS KPSNMLQTSD CLGADTLIKL 

       250        260        270        280        290        300 
LKNYSRSHEL KKSITVGIIG LPNVGKSSLI NSLKRAHVVN VGATPGLTRS LQEVHLDKNV 

       310        320        330        340        350        360 
KLLDCPGVVM LKSSGNDASI ALRNCKRIEK LDDPVSPVKE ILKLCPKDML VTLYKIPSFE 

       370        380        390        400        410        420 
AVDDFLYKVA TVRGKLKKGG LVDIDAAARI VLHDWNEGKI PYYTMPPKRD QGGHAESKIV 

       430        440        450        460        470        480 
TELAKDFNID EVYSGESSFI GSLKTVNEFN PVIIPSNGPL NFDETMIEDE SKTQTEEEAE 

       490        500        510        520        530        540 
HESDDDESMG GEEEEEAGKT KEKSETGRQN VKLYAAESML NTKKQKAEKK KRKKAKKAGA 

       550        560        570        580 
DEEDLMDGDY DFKVDYRKNK DGEDEEFQID AKIPMAGLLP EE 

References and documentation are available.

Number of amino acids: 582

Molecular weight: 65785.53

Theoretical pI: 8.67

Amino acid composition: 
Ala (A)  37	  6.4%
Arg (R)  29	  5.0%
Asn (N)  23	  4.0%
Asp (D)  43	  7.4%
Cys (C)   6	  1.0%
Gln (Q)  13	  2.2%
Glu (E)  60	 10.3%
Gly (G)  32	  5.5%
His (H)  11	  1.9%
Ile (I)  26	  4.5%
Leu (L)  56	  9.6%
Lys (K)  81	 13.9%
Met (M)  14	  2.4%
Phe (F)  12	  2.1%
Pro (P)  25	  4.3%
Ser (S)  35	  6.0%
Thr (T)  23	  4.0%
Trp (W)   4	  0.7%
Tyr (Y)  11	  1.9%
Val (V)  41	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 110

Atomic composition:

Carbon      C	      2888
Hydrogen    H	      4742
Nitrogen    N	       812
Oxygen      O	       894
Sulfur      S	        20

Formula: C2888H4742N812O894S20
Total number of atoms: 9356

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38765
Abs 0.1% (=1 g/l)   0.589, assuming all pairs of Cys residues form cystines


Ext. coefficient    38390
Abs 0.1% (=1 g/l)   0.584, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.15
This classifies the protein as unstable.



Aliphatic index: 81.74

Grand average of hydropathicity (GRAVY): -0.754