ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKRTVATNG DASGAHRAKK MSKTHASHII NAQEDYKHMY LSVQPLDIFC WGTGSMCELG 

        70         80         90        100        110        120 
LGPLAKNKEV KRPRLNPFLP RDEAKIISFA VGGMHTLALD EESNVWSWGC NDVGALGRDT 

       130        140        150        160        170        180 
SNAKEQLKDM DADDSSDDED GDLNELESTP AKIPRESFPP LAEGHKVVQL AATDNMSCAL 

       190        200        210        220        230        240 
FSNGEVYAWG TFRCNEGILG FYQDKIKIQK TPWKVPTFSK YNIVQLAPGK DHILFLDEEG 

       250        260        270        280        290        300 
MVFAWGNGQQ NQLGRKVMER FRLKTLDPRP FGLRHVKYIA SGENHCFALT KDNKLVSWGL 

       310        320        330        340        350        360 
NQFGQCGVSE DVEDGALVTK PKRLALPDNV VIRSIAAGEH HSLILSQDGD LYSCGRLDMF 

       370        380        390        400        410        420 
EVGIPKDNLP EYTYKDVHGK ARAVPLPTKL NNVPKFKSVA AGSHHSVAVA QNGIAYSWGF 

       430        440        450        460        470        480 
GETYAVGLGP FEDDTEVPTR IKNTATQDHN IILVGCGGQF SVSGGVKLSD EDAEKRADEM 


DD 

References and documentation are available.

Number of amino acids: 482

Molecular weight: 53013.82

Theoretical pI: 5.71

Amino acid composition: 
Ala (A)  38	  7.9%
Arg (R)  19	  3.9%
Asn (N)  24	  5.0%
Asp (D)  37	  7.7%
Cys (C)   9	  1.9%
Gln (Q)  16	  3.3%
Glu (E)  29	  6.0%
Gly (G)  44	  9.1%
His (H)  15	  3.1%
Ile (I)  20	  4.1%
Leu (L)  40	  8.3%
Lys (K)  35	  7.3%
Met (M)  11	  2.3%
Phe (F)  19	  3.9%
Pro (P)  23	  4.8%
Ser (S)  30	  6.2%
Thr (T)  21	  4.4%
Trp (W)   8	  1.7%
Tyr (Y)  11	  2.3%
Val (V)  33	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2339
Hydrogen    H	      3646
Nitrogen    N	       652
Oxygen      O	       717
Sulfur      S	        20

Formula: C2339H3646N652O717S20
Total number of atoms: 7374

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60890
Abs 0.1% (=1 g/l)   1.149, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.139, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.14
This classifies the protein as stable.



Aliphatic index: 76.29

Grand average of hydropathicity (GRAVY): -0.436