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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ

70 80 90 100 110 120
SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH GFHEKELSKY FAQFGDLKEV

130 140 150 160 170 180
RLARNKKTGN SRHYGFLEFV NKEDAMIAQE SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK

190 200 210 220
KRVLVEKGIT KPVKQLKDNM KQKHEERIKK LAKSGIEFKW


References and documentation are available.

Number of amino acids: 220 Molecular weight: 25447.07 Theoretical pI: 9.26
Amino acid composition: 
Ala (A) 10 4.5% Arg (R) 8 3.6% Asn (N) 8 3.6% Asp (D) 10 4.5% Cys (C) 0 0.0% Gln (Q) 9 4.1% Glu (E) 28 12.7% Gly (G) 11 5.0% His (H) 6 2.7% Ile (I) 10 4.5% Leu (L) 19 8.6% Lys (K) 38 17.3% Met (M) 5 2.3% Phe (F) 6 2.7% Pro (P) 6 2.7% Ser (S) 17 7.7% Thr (T) 11 5.0% Trp (W) 1 0.5% Tyr (Y) 7 3.2% Val (V) 10 4.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 38 Total number of positively charged residues (Arg + Lys): 46 Atomic composition: Carbon C 1123 Hydrogen H 1830 Nitrogen N 312 Oxygen O 349 Sulfur S 5 Formula: C1123H1830N312O349S5 Total number of atoms: 3619 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15930 Abs 0.1% (=1 g/l) 0.626 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.76 This classifies the protein as unstable. Aliphatic index: 69.14 Grand average of hydropathicity (GRAVY): -1.080