ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV 

        70         80         90        100        110        120 
SKLSDGLPPG ASDARGAKPA ECTYLEAHKD TDLLAVGYAD GVIKVWDLMS KTVLLNFNGH 

       130        140        150        160        170        180 
KAAITLLQFD GTGTRLISGS KDSNIIVWDL VGEVGLYKLR SHKDSITGFW CQGEDWLIST 

       190        200        210        220        230        240 
SKDGMIKLWD LKTHQCIETH IAHTGECWGL AVKDDLLITT GTDSQVKIWK LDIENDKMGG 

       250        260        270        280        290        300 
KLTEMGIFEK QSKQRGLKIE FITNSSDKTS FFYIQNADKT IETFRIRKEE EIARGLKKRE 

       310        320        330        340        350        360 
KRLKEKGLTE EEIAKSIKES YSSFILHPFQ TIRSLYKIKS ASWTTVSSSK LELVLTTSSN 

       370        380        390        400        410        420 
TIEYYSIPYE KRDPTSPAPL KTHTIELQGQ RTDVRSIDIS DDNKLLATAS NGSLKIWNIK 

       430        440        450        460        470        480 
THKCIRTFEC GYALTCKFLP GGLLVILGTR NGELQLFDLA SSSLLDTIED AHDAAIWSLD 

       490        500        510        520        530        540 
LTSDGKRLVT GSADKTVKFW DFKVENSLVP GTKNKFLPVL KLHHDTTLEL TDDILCVRVS 

       550        560        570        580        590        600 
PDDRYLAISL LDNTVKVFFL DSMKFYLSLY GHKLPVLSID ISFDSKMIIT SSADKNIKIW 

       610        620        630        640        650        660 
GLDFGDCHKS LFAHQDSIMN VKFLPQSHNF FSCSKDAVVK YWDGEKFECI QKLYAHQSEV 

       670        680        690        700        710        720 
WALAVATDGG FVVSSSHDHS IRIWEETEDQ VFLEEEKEKE LEEQYEDTLL TSLEEGNGDD 

       730        740        750        760        770        780 
AFKADASGEG VEDEASGVHK QTLESLKAGE RLMEALDLGI AEIEGLEAYN RDMKLWQRKK 

       790        800        810        820        830        840 
LGEAPIKPQG NAVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM 

       850        860        870        880        890        900 
QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRSA LKSTEDDLGF 

       910        920        930        940 
NVQGLKFVKQ QWNLRHNYEF VDEYDQQEKE SNSARKRVFG TVI 

References and documentation are available.

Number of amino acids: 943

Molecular weight: 106342.25

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  53	  5.6%
Arg (R)  30	  3.2%
Asn (N)  35	  3.7%
Asp (D)  68	  7.2%
Cys (C)  13	  1.4%
Gln (Q)  33	  3.5%
Glu (E)  68	  7.2%
Gly (G)  58	  6.2%
His (H)  24	  2.5%
Ile (I)  65	  6.9%
Leu (L) 105	 11.1%
Lys (K)  85	  9.0%
Met (M)  15	  1.6%
Phe (F)  41	  4.3%
Pro (P)  21	  2.2%
Ser (S)  79	  8.4%
Thr (T)  59	  6.3%
Trp (W)  19	  2.0%
Tyr (Y)  22	  2.3%
Val (V)  50	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 136
Total number of positively charged residues (Arg + Lys): 115

Atomic composition:

Carbon      C	      4764
Hydrogen    H	      7511
Nitrogen    N	      1253
Oxygen      O	      1444
Sulfur      S	        28

Formula: C4764H7511N1253O1444S28
Total number of atoms: 15000

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   138030
Abs 0.1% (=1 g/l)   1.298, assuming all pairs of Cys residues form cystines


Ext. coefficient   137280
Abs 0.1% (=1 g/l)   1.291, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.24
This classifies the protein as unstable.



Aliphatic index: 91.30

Grand average of hydropathicity (GRAVY): -0.309