ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKTEICGKG SSQGSEEERL SQLPDHLICV ILSHLSTKDA VRTSILSTRW RNLWQLVPVL
70 80 90 100 110 120
DFDSRELRSF SEFVSFAGSF FYLHKDSYIQ KLRVCIYDLA GNYYLTSWID LVTRHRIQHI
130 140 150 160 170 180
DISVFTCSGF GVIPLSLYTC DTLVHLKLSR VTMVNVEFVS LPCLKILDLD FVNFTNETTL
190 200 210 220 230 240
DKIISCSPVL EELTIVKSSE DNVKIIQVRS QTLKRVEIHR RFDRHNGLVI DTPLLQFLSI
250 260 270 280 290 300
KAHSIKSIEF INLGFTTKVD IDVNLLDPND LSNRSMTRDF FTTISRVRSL VIRHGTIKDI
310 320 330 340 350 360
FHYMELEPLQ QFCYLSELSA VCSISNLEML LNLLKSCPKL ESLSLKLVDY EKNKKEEVMS
370 380 390 400 410 420
STVPPPCLVS SLKFVKLESQ LLGCGTELKV ARYFLENSTI LEKLTLKIDY MYKDEANVNH
430 440
IRQTLHAVPR CSSTCEVVIH SLLY
References and
documentation are available.
Number of amino acids: 444
Molecular weight: 50907.18
Theoretical pI: 6.91
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2289
Hydrogen H 3668
Nitrogen N 600
Oxygen O 665
Sulfur S 21
Formula: C
2289H
3668N
600O
665S
21
Total number of atoms: 7243
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36745
Abs 0.1% (=1 g/l) 0.722, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.705, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.46
This classifies the protein as unstable.
Aliphatic index: 110.29
Grand average of hydropathicity (GRAVY): 0.063