ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKTNISKNS PKEATVPELD WPFKLIKSHL ETRKLETENV WIACFEPKYA SKVTQYVKQI
70 80 90 100 110 120
RSKQKESLLH CNRLRRIQDE NGSLELQIII CPEKSMTANE IGKDFEDLGI VSKMIFLYAV
130 140 150 160 170 180
PAFPPLTDEQ FHEWNSVWPV SYRKHVQRQD VFTVHELKRI ESILEDLINA AGASHKHGEI
190 200 210 220 230 240
GCAAAIYDPT TDTVLAVSVD ERSKLKNPIN HCVMNAINLV AKRELSRRQN RTDGSKDRYL
250 260 270 280 290 300
CKDLTVVMTH EPCVMCSMGL LHSRIRRLIY CKKQPLTGGI ESLYGIHWRA ELNHRYLAYS
310
GWNKPVPSIK ENIHV
References and
documentation are available.
Number of amino acids: 315
Molecular weight: 36275.92
Theoretical pI: 8.93
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1613
Hydrogen H 2574
Nitrogen N 456
Oxygen O 463
Sulfur S 16
Formula: C
1613H
2574N
456O
463S
16
Total number of atoms: 5122
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48400
Abs 0.1% (=1 g/l) 1.334, assuming all pairs of Cys residues form cystines
Ext. coefficient 47900
Abs 0.1% (=1 g/l) 1.320, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.41
This classifies the protein as unstable.
Aliphatic index: 90.03
Grand average of hydropathicity (GRAVY): -0.417