ProtParam
User-provided sequence:
10 20 30 40 50 60
MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV
70 80 90 100 110 120
VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV
130 140 150 160 170 180
NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG
190 200 210 220 230 240
WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF
250 260 270 280 290 300
CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN
310 320 330 340 350 360
DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK
370 380 390 400 410 420
VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN
430 440 450 460 470 480
GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD
490 500 510 520 530 540
TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE
550
IVSTKATPTD LSKL
References and
documentation are available.
Number of amino acids: 554
Molecular weight: 62791.13
Theoretical pI: 6.72
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2821
Hydrogen H 4437
Nitrogen N 761
Oxygen O 819
Sulfur S 21
Formula: C
2821H
4437N
761O
819S
21
Total number of atoms: 8859
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72685
Abs 0.1% (=1 g/l) 1.158, assuming all pairs of Cys residues form cystines
Ext. coefficient 72310
Abs 0.1% (=1 g/l) 1.152, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.09
This classifies the protein as stable.
Aliphatic index: 90.99
Grand average of hydropathicity (GRAVY): -0.303