ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLADLGKRI NSAVNNAISN TQDDFTTSVD VMLKGIVTAL LESDVNIALV SKLRNNIRSQ
70 80 90 100 110 120
LLSENRSEKS TTNAQTKKLI QKTVFDELCK LVTCEGSEEK AFVPKKRKTN IIMFVGLQGS
130 140 150 160 170 180
GKTTSCTKLA VYYSKRGFKV GLVCADTFRA GAFDQLKQNA IRARIPFYGS YTETDPAKVA
190 200 210 220 230 240
EEGINKFKKE KFDIIIVDTS GRHHQEEELF QEMIEISNVI KPNQTIMVLD ASIGQAAEQQ
250 260 270 280 290 300
SKAFKESSDF GAIILTKMDG HARGGGAISA VAATNTPIIF IGTGEHIHDL EKFSPKSFIS
310 320 330 340 350 360
KLLGIGDIES LFEQLQTVSN KEDAKATMEN IQKGKFTLLD FKKQMQTIMK MGPLSNIAQM
370 380 390 400 410 420
IPGMSNMMNQ VGEEETSQKM KKMVYVLDSM TKEELESDGR MFIEEPTRMV RVAKGSGTSV
430 440 450 460 470 480
FEVEMILMQQ QMMARMAQTA TQQQPGAPGA NARMPGMPNM PGMPNMPGMP NMPGMPKVTP
490 500 510 520 530 540
QMMQQAQQKL KQNPGLMQNM MNMFGGGMGG GMGGGMPDMN EMMKMMQDPQ MQQMAKQFGM
G
References and
documentation are available.
Number of amino acids: 541
Molecular weight: 59624.27
Theoretical pI: 8.73
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2579
Hydrogen H 4209
Nitrogen N 713
Oxygen O 795
Sulfur S 53
Formula: C
2579H
4209N
713O
795S
53
Total number of atoms: 8349
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7700
Abs 0.1% (=1 g/l) 0.129, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.125, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.42
This classifies the protein as unstable.
Aliphatic index: 70.15
Grand average of hydropathicity (GRAVY): -0.343