ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVLAMESRVA PEIPGLIQPG NVTQDLKMMV CKLLNSPKPT KTFPGSQPVS FQHSDVEEKL 

        70         80         90        100        110        120 
LAHDYYVCEK TDGLRVLMFI VINPVTGEQG CFMIDRENNY YLVNGFRFPR LPQKKKEELL 

       130        140        150        160        170        180 
ETLQDGTLLD GELVIQTNPM TKLQELRYLM FDCLAINGRC LTQSPTSSRL AHLGKEFFKP 

       190        200        210        220        230        240 
YFDLRAAYPN RCTTFPFKIS MKHMDFSYQL VKVAKSLDKL PHLSDGLIFT PVKAPYTAGG 

       250        260        270        280        290        300 
KDSLLLKWKP EQENTVDFKL ILDIPMVEDP SLPKDDRNRW YYNYDVKPVF SLYVWQGGAD 

       310        320        330        340        350        360 
VNSRLKHFDQ PFDRKEFEIL ERTYRKFAEL SVSDEEWQNL KNLEQPLNGR IVECAKNQET 

       370        380        390        400        410        420 
GAWEMLRFRD DKLNGNHTSV VQKVLESIND SVSLEDLEEI VGDIKRCWDE RRANMAGGSG 

       430        440        450 
RPLPSQSQNA TLSTSKPVHS QPPSNDKEPK YVDEDDWSD 

References and documentation are available.

Number of amino acids: 459

Molecular weight: 52764.16

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  17	  3.7%
Arg (R)  23	  5.0%
Asn (N)  23	  5.0%
Asp (D)  34	  7.4%
Cys (C)   8	  1.7%
Gln (Q)  21	  4.6%
Glu (E)  33	  7.2%
Gly (G)  23	  5.0%
His (H)   8	  1.7%
Ile (I)  16	  3.5%
Leu (L)  50	 10.9%
Lys (K)  35	  7.6%
Met (M)  13	  2.8%
Phe (F)  21	  4.6%
Pro (P)  31	  6.8%
Ser (S)  30	  6.5%
Thr (T)  21	  4.6%
Trp (W)   7	  1.5%
Tyr (Y)  15	  3.3%
Val (V)  30	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2356
Hydrogen    H	      3676
Nitrogen    N	       630
Oxygen      O	       704
Sulfur      S	        21

Formula: C2356H3676N630O704S21
Total number of atoms: 7387

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61350
Abs 0.1% (=1 g/l)   1.163, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   1.153, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.60
This classifies the protein as unstable.



Aliphatic index: 78.74

Grand average of hydropathicity (GRAVY): -0.556