ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVLETLKQGL DSSQIHEALI QLDSYPREPV DLDASMVLIK FVIPVYPSLP ERSKVILRRL 

        70         80         90        100        110        120 
ASKSFTFLCQ IVTFSRTISG RDGLQEIRIY QEILEDIISF EPGCLTFYLK ASTTSKADRD 

       130        140        150        160        170        180 
SIKALFFGSK LFNVLANRID MAKYLGYLRL QWKFLLESNE TDPPGFLGEW LVSSFLLNPV 

       190        200        210        220        230        240 
LAADMLLGEL FLLKESYFFS FQKIISASSL IDQKRLIAKF LLPYIQVIVT LENLNDVRKI 

       250        260        270        280        290        300 
LRRFDLDKII SLSVLFEIQS LPLKEVIVRL MSNHSSTKFV SALVSKFADF TDEEVDTKTC 

       310        320        330        340        350        360 
ELLVLFAVHN LNHSQREEIA HDERFLNGVT KHLGSNEREA RERAMFIAKL LSGGHLKYES 

       370        380        390        400        410        420 
DFKINIPNVK FESNSDDKII DFQSLKNPSI CNTQTDVGKD KITEVSGHVQ SLTLDCSDSD 

       430        440        450        460        470        480 
DEDENDEREI VKRIVFLKDL MKEYEKTGES RKAPLIPLLK QTVKLIRQKA DFQLEVGYYA 

       490        500        510        520        530        540 
QGILSSIVCL NNEFDEPLFE QWRINALTSI LVVLPEKVNG AINILFNSEL SLQQRMSLLS 

       550        560        570        580        590        600 
ALGLSARELR GLDDPTIVKP KFDFPTNRLP WDDQSHHNSR LVEVQESTSM IKKTKTVWKS 

       610        620        630        640        650        660 
RKLGKDREKG TQNRFRKYAG LFFYPLAHGW LNGIDVGTYN QLFKSHYLTT LRIIYSCANP 

       670        680 
VHDFESMTEL MNHIISSAIE EGISLNKG 

References and documentation are available.

Number of amino acids: 688

Molecular weight: 78686.68

Theoretical pI: 6.22

Amino acid composition: 
Ala (A)  30	  4.4%
Arg (R)  35	  5.1%
Asn (N)  31	  4.5%
Asp (D)  42	  6.1%
Cys (C)   7	  1.0%
Gln (Q)  26	  3.8%
Glu (E)  49	  7.1%
Gly (G)  29	  4.2%
His (H)  14	  2.0%
Ile (I)  52	  7.6%
Leu (L)  92	 13.4%
Lys (K)  50	  7.3%
Met (M)  11	  1.6%
Phe (F)  40	  5.8%
Pro (P)  23	  3.3%
Ser (S)  63	  9.2%
Thr (T)  31	  4.5%
Trp (W)   6	  0.9%
Tyr (Y)  17	  2.5%
Val (V)  40	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      3556
Hydrogen    H	      5648
Nitrogen    N	       934
Oxygen      O	      1039
Sulfur      S	        18

Formula: C3556H5648N934O1039S18
Total number of atoms: 11195

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58705
Abs 0.1% (=1 g/l)   0.746, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.08
This classifies the protein as stable.



Aliphatic index: 102.85

Grand average of hydropathicity (GRAVY): -0.156