ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLKSTSAND VSVYQVSGTN VSRSLPDWIA KKRKRQLKND LEYQNRVELI QDFEFSEASN
70 80 90 100 110 120
KIKVSRDGQY CMATGTYKPQ IHVYDFANLS LKFDRHTDAE NVDFTILSDD WTKSVHLQND
130 140 150 160 170 180
RSIQFQNKGG LHYTTRIPKF GRSLVYNKVN CDLYVGASGN ELYRLNLEKG RFLNPFKLDT
190 200 210 220 230 240
EGVNHVSINE VNGLLAAGTE TNVVEFWDPR SRSRVSKLYL ENNIDNRPFQ VTTTSFRNDG
250 260 270 280 290 300
LTFACGTSNG YSYIYDLRTS EPSIIKDQGY GFDIKKIIWL DNVGTENKIV TCDKRIAKIW
310 320 330 340 350 360
DRLDGKAYAS MEPSVDINDI EHVPGTGMFF TANESIPMHT YYIPSLGPSP RWCSFLDSIT
370 380 390 400 410 420
EELEEKPSDT VYSNYRFITR DDVKKLNLTH LVGSRVLRAY MHGFFINTEL YDKVSLIANP
430 440 450 460 470 480
DAYKDERERE IRRRIEKERE SRIRSSGAVQ KPKIKVNKTL VDKLSQKRGD KVAGKVLTDD
490 500 510 520 530 540
RFKEMFEDEE FQVDEDDYDF KQLNPVKSIK ETEEGAAKRI RALTAAEESD EERIAMKDGR
550 560 570 580 590 600
GHYDYEDEES DEEESDDETN QKSNKEELSE KDLRKMEKQK ALIERRKKEK EQSERFMNEM
610 620 630 640 650 660
KAGTSTSTQR DESAHVTFGE QVGELLEVEN GKKSNESILR RNQRGEAELT FIPQRKSKKD
670 680 690 700
GNYKSRRHDN SSDEEGIEEN GNKKDNGRSK PRFENRRRAS KNAFRGM
References and
documentation are available.
Number of amino acids: 707
Molecular weight: 81748.05
Theoretical pI: 6.20
Total number of negatively charged residues (Asp + Glu): 124
Total number of positively charged residues (Arg + Lys): 118
Atomic composition:
Carbon C 3559
Hydrogen H 5623
Nitrogen N 1033
Oxygen O 1145
Sulfur S 17
Formula: C
3559H
5623N
1033O
1145S
17
Total number of atoms: 11377
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71990
Abs 0.1% (=1 g/l) 0.881, assuming all pairs of Cys residues form cystines
Ext. coefficient 71740
Abs 0.1% (=1 g/l) 0.878, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.40
This classifies the protein as unstable.
Aliphatic index: 66.17
Grand average of hydropathicity (GRAVY): -0.967