ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF
70 80 90 100 110 120
QIFRKLFSRG DLTLPSSKKS TLEKEQFVNW CRKVYEAFKT KLLAIISDIP FETSLGLDSL
130 140 150 160 170 180
DVYLQLAELE STHFASEKGA PFFPNKTFRK LIIALWSSNM GEIEDVKSSG ASENLIIVEF
190 200 210 220 230 240
TEKYYTKFAD IQYYFQSEFN QLLEDPAYQD LLLKNVGKWL ALVNHDKHCS SVDADLEIFV
250 260 270 280 290 300
PNPPQAIENE SKFKSNFEKN WLSLLNGQLS LQQYKSILLI LHKRIIPHFH TPTKLMDFLT
310 320 330 340 350 360
DSYNLQSSNK NAGVVPILAL NGLFELMKRF NLEYPNFYMK LYQIINPDLM HVKYRARFFR
370 380 390 400 410 420
LMDVFLSSTH LSAHLVASFI KKLARLTLES PPSAIVTVIP FIYNLIRKHP NCMIMLHNPA
430 440 450 460 470 480
FISNPFQTPD QVANLKTLKE NYVDPFDVHE SDPELTHALD SSLWELASLM EHYHPNVATL
490 500 510 520 530 540
AKIFAQPFKK LSYNMEDFLD WNYDSLLNAE SSRKLKTLPT LEFEAFTNVF DNEDGDSEAS
550
SQGNVYLPGV AW
References and
documentation are available.
Number of amino acids: 552
Molecular weight: 63637.15
Theoretical pI: 5.74
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2918
Hydrogen H 4504
Nitrogen N 730
Oxygen O 834
Sulfur S 15
Formula: C
2918H
4504N
730O
834S
15
Total number of atoms: 9001
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68425
Abs 0.1% (=1 g/l) 1.075, assuming all pairs of Cys residues form cystines
Ext. coefficient 68300
Abs 0.1% (=1 g/l) 1.073, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.40
This classifies the protein as stable.
Aliphatic index: 97.17
Grand average of hydropathicity (GRAVY): -0.173