ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLPLLQRST LRGVQQMTKP WAAIGSLRLA SQEFRVESDT FGELKVPADK YYGAQTMRSQ
70 80 90 100 110 120
INFPIGGATE RMPKPVVQAM GILKKAAAEV NKEFGLDSKV SEAISKAADD VISGKLYDDH
130 140 150 160 170 180
FPLVIWQTGS GTQSNMNVNE VISNRAIELL GGKLGSKTPV HPNDHVNKSQ SSNDTFPTAI
190 200 210 220 230 240
HISVALELNN NLKPAIKTLH DALRAKSEEF KDIIKIGRTH TMDAVPLTLG QEFSGYAQQL
250 260 270 280 290 300
AYAQERIDAC LPRVYELALG GTAVGTGLNT RKGFAEKCAA KIAELTSLPF VTAPNKFEAL
310 320 330 340 350 360
AARDAMVEVH GVLNTIAVSL MKIANDIRFL GSGPRCGLGE LSLPENEPGS SIMPGKVNPT
370 380 390 400 410 420
QCESLTMLSA QVMGNQVAVT IGGSNGHFEL NVFKPLIVSN VLRSIRLLSD GSRTFTANCV
430 440 450 460 470 480
NGIQANRENI AKIMNESLML VTALNPHIGY DKAAKIAKTA HKNGTTLKEE AINLGYLTEQ
490
QFNDWVRPEQ MLGPK
References and
documentation are available.
Number of amino acids: 495
Molecular weight: 53575.54
Theoretical pI: 8.61
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2362
Hydrogen H 3822
Nitrogen N 662
Oxygen O 715
Sulfur S 20
Formula: C
2362H
3822N
662O
715S
20
Total number of atoms: 7581
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28670
Abs 0.1% (=1 g/l) 0.535, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.530, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.73
This classifies the protein as stable.
Aliphatic index: 91.27
Grand average of hydropathicity (GRAVY): -0.160