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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVLPLLQRST LRGVQQMTKP WAAIGSLRLA SQEFRVESDT FGELKVPADK YYGAQTMRSQ

70 80 90 100 110 120
INFPIGGATE RMPKPVVQAM GILKKAAAEV NKEFGLDSKV SEAISKAADD VISGKLYDDH

130 140 150 160 170 180
FPLVIWQTGS GTQSNMNVNE VISNRAIELL GGKLGSKTPV HPNDHVNKSQ SSNDTFPTAI

190 200 210 220 230 240
HISVALELNN NLKPAIKTLH DALRAKSEEF KDIIKIGRTH TMDAVPLTLG QEFSGYAQQL

250 260 270 280 290 300
AYAQERIDAC LPRVYELALG GTAVGTGLNT RKGFAEKCAA KIAELTSLPF VTAPNKFEAL

310 320 330 340 350 360
AARDAMVEVH GVLNTIAVSL MKIANDIRFL GSGPRCGLGE LSLPENEPGS SIMPGKVNPT

370 380 390 400 410 420
QCESLTMLSA QVMGNQVAVT IGGSNGHFEL NVFKPLIVSN VLRSIRLLSD GSRTFTANCV

430 440 450 460 470 480
NGIQANRENI AKIMNESLML VTALNPHIGY DKAAKIAKTA HKNGTTLKEE AINLGYLTEQ

490
QFNDWVRPEQ MLGPK


References and documentation are available.

Number of amino acids: 495 Molecular weight: 53575.54 Theoretical pI: 8.61
Amino acid composition: 
Ala (A) 48 9.7% Arg (R) 20 4.0% Asn (N) 31 6.3% Asp (D) 18 3.6% Cys (C) 5 1.0% Gln (Q) 21 4.2% Glu (E) 30 6.1% Gly (G) 39 7.9% His (H) 10 2.0% Ile (I) 30 6.1% Leu (L) 49 9.9% Lys (K) 32 6.5% Met (M) 15 3.0% Phe (F) 16 3.2% Pro (P) 24 4.8% Ser (S) 33 6.7% Thr (T) 30 6.1% Trp (W) 3 0.6% Tyr (Y) 8 1.6% Val (V) 33 6.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 48 Total number of positively charged residues (Arg + Lys): 52 Atomic composition: Carbon C 2362 Hydrogen H 3822 Nitrogen N 662 Oxygen O 715 Sulfur S 20 Formula: C2362H3822N662O715S20 Total number of atoms: 7581 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 28670 Abs 0.1% (=1 g/l) 0.535, assuming all pairs of Cys residues form cystines Ext. coefficient 28420 Abs 0.1% (=1 g/l) 0.530, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 31.73 This classifies the protein as stable. Aliphatic index: 91.27 Grand average of hydropathicity (GRAVY): -0.160