ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLQKLPLIG LLLLLTIVAS PANADGPVCP PSNKLSRASF PEGFLFGTAT AAYQVEGAIN
70 80 90 100 110 120
ETCRGPALWD IYCRRYPERC NNDNGDVAVD FFHRYKEDIQ LMKNLNTDAF RMSIAWPRIF
130 140 150 160 170 180
PHGRKEKGVS QAGVQFYHDL IDELIKNGIT PFVTVFHWDT PQDLEDEYGG FLSERIVKDF
190 200 210 220 230 240
REYADFVFQE YGGKVKHWIT FNEPWVFSHA GYDVGKKAPG RCSSYVNAKC QDGRSGYEAY
250 260 270 280 290 300
LVTHNLLISH AEAVEAYRKC EKCKGGKIGI AHSPAWFEAH DLADSQDGAS IDRALDFILG
310 320 330 340 350 360
WHLDTTTFGD YPQIMKDIVG HRLPKFTTEQ KAKLKASTDF VGLNYYTSVF SNHLEKPDPS
370 380 390 400 410 420
KPRWMQDSLI TWESKNAQNY AIGSKPLTAA LNVYSRGFRS LLKYIKDKYA NPEIMIMENG
430 440 450 460 470 480
YGEELGASDS VAVGTADHNR KYYLQRHLLS MQEAVCIDKV NVTGYFVWSL LDNFEWQDGY
490 500 510 520
KNRFGLYYVD FKNNLTRYEK ESGKYYKDFL SQGVRPSALK KDEL
References and
documentation are available.
Number of amino acids: 524
Molecular weight: 59720.57
Theoretical pI: 6.45
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2706
Hydrogen H 4087
Nitrogen N 717
Oxygen O 782
Sulfur S 17
Formula: C
2706H
4087N
717O
782S
17
Total number of atoms: 8309
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 105700
Abs 0.1% (=1 g/l) 1.770, assuming all pairs of Cys residues form cystines
Ext. coefficient 105200
Abs 0.1% (=1 g/l) 1.762, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.14
This classifies the protein as stable.
Aliphatic index: 75.40
Grand average of hydropathicity (GRAVY): -0.442