ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLRIRRIKK LAPLIFTSLL SLIVLFRVYR QYPFSDHFET RREDDRSGNV HCFSRLRQIE
70 80 90 100 110 120
EYEKPELTSK FYEPNRWKSF ISYVTRSRKD VKTVSRSLSN LDLYQKCSKE IRADQDISLL
130 140 150 160 170 180
HSIETKLFPY INFTALNSEQ SHNFWPVHTR FDGTKYRGQV LQFSSENNSF IGTSPIEFKA
190 200 210 220 230 240
SEPFWENWLN SALQRNSKGV VMSVSEYLVA DTIRLIRVLR LLNNSLPIEI VHKSDLHESS
250 260 270 280 290 300
QQLLIAAARE SGSLNYPPQE LWFLDVKDML NDEYLARFKR FSNKWLAITF CSFQIPIFLD
310 320 330 340 350 360
SDTVPFVPLD TLYEIDGFKR TGTLFFKDRS FPTSKLSPLQ VKVLKQIINN SLDVSSDSEQ
370 380 390 400 410 420
GFEILKHNLN DEMAIDAIEA LIFKKQKHYM DSGLVIFDKQ KHFFCLPIAI MLQFSPIQEF
430 440 450 460 470 480
FHGDKEWFWL SLFISKKEFT FYPIEASNVG RLEKPETLES STICSTQLSH TDVYGNLLWL
490 500 510 520 530 540
NGGLSVCKKN CWNYDFTKRK EIAAKYKSVD ELRNYYQSPV KLEAVIIPDV SKSPWSQQSE
550 560 570 580
CVMYSYCTHY RKGQYGKLIE FTDSQKKYYE KVVELWNKVV
References and
documentation are available.
Number of amino acids: 580
Molecular weight: 68082.01
Theoretical pI: 8.67
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 3111
Hydrogen H 4780
Nitrogen N 806
Oxygen O 881
Sulfur S 16
Formula: C
3111H
4780N
806O
881S
16
Total number of atoms: 9594
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 103750
Abs 0.1% (=1 g/l) 1.524, assuming all pairs of Cys residues form cystines
Ext. coefficient 103250
Abs 0.1% (=1 g/l) 1.517, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.84
This classifies the protein as unstable.
Aliphatic index: 86.84
Grand average of hydropathicity (GRAVY): -0.352