ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLRNLTISY EIIKSLKHAK EHSLGIQIRW ISSLKVVWKK DTRLDEAIEQ DKRYKLCARV
70 80 90 100 110 120
VKEVLNEPGQ VIPLRYLEKR RERLRLTFKA KSFVEMNPSL FEIYYDRIKP KSDPVQFLRP
130 140 150 160 170 180
TPRLRAFLDE EQRIYSENEP LLVSKLCQLL MMAKDKVISA DKLVHVKRDF GFPNDFLMKL
190 200 210 220 230 240
VQKYPNYFRL TGLPEEGKSF LELVSWNPDF AKSKIELRAE DETLKTGVRI RPNYNVKLPS
250 260 270 280 290 300
GFFLRKEMRE WTRDWLEQDY ISPYEDVSHL DQASKEMEKR TVGVVHELLS LSLLKRVPVP
310 320 330 340 350 360
TLGKFCDEFR FSNAFSSVFT RHSGIFYLSL KGGIKTAVLR EAYKDDELVD RDPLLAIKDK
370 380 390 400
FLRLLEEGWQ ERKDRLKLQR EQVQKDREMM LAAKSNEPIE RLCD
References and
documentation are available.
Number of amino acids: 404
Molecular weight: 47810.61
Theoretical pI: 9.32
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2162
Hydrogen H 3457
Nitrogen N 591
Oxygen O 604
Sulfur S 13
Formula: C
2162H
3457N
591O
604S
13
Total number of atoms: 6827
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52620
Abs 0.1% (=1 g/l) 1.101, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 1.095, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.97
This classifies the protein as stable.
Aliphatic index: 92.85
Grand average of hydropathicity (GRAVY): -0.516