ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLSDKELFA INKKAVEQGF NVKPRLNYNT VSGVNGPLVI LEKVKFPRYN EIVNLTLPDG
70 80 90 100 110 120
TVRQGQVLEI RGDRAIVQVF EGTSGIDVKK TTVEFTGESL RIPVSEDMLG RIFDGSGRPI
130 140 150 160 170 180
DNGPKVFAED YLDINGSPIN PYARIYPEEM ISTGVSAIDT MNSIARGQKI PIFSASGLPH
190 200 210 220 230 240
NEIAAQICRQ AGLVRPTKDV HDGHEENFSI VFAAMGVNLE TARFFKQDFE ENGSLERTSL
250 260 270 280 290 300
FLNLANDPTI ERIITPRLAL TTAEYLAYQT ERHVLTILTD MSSYADALRE VSAAREEVPG
310 320 330 340 350 360
RRGYPGYMYT DLSTIYERAG RVEGRNGSIT QIPILTMPND DITHPIPDLT GYITEGQIFV
370 380 390 400 410 420
DRQLHNKGIY PPINVLPSLS RLMKSAIGEG MTRKDHGDVS NQLYAKYAIG KDAAAMKAVV
430 440 450 460 470 480
GEEALSIEDK LSLEFLEKFE KTFITQGAYE DRTVFESLDQ AWSLLRIYPK EMLNRISPKI
490 500 510
LDEFYDRARD DADEDEEDPD TRSSGKKKDA SQEESLI
References and
documentation are available.
Number of amino acids: 517
Molecular weight: 57749.25
Theoretical pI: 4.95
Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2556
Hydrogen H 4052
Nitrogen N 696
Oxygen O 800
Sulfur S 13
Formula: C
2556H
4052N
696O
800S
13
Total number of atoms: 8117
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33810
Abs 0.1% (=1 g/l) 0.585, assuming all pairs of Cys residues form cystines
Ext. coefficient 33810
Abs 0.1% (=1 g/l) 0.585, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.72
This classifies the protein as stable.
Aliphatic index: 87.72
Grand average of hydropathicity (GRAVY): -0.378