ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLSDSLKLP SPTLSAAAGV DDCDGEDHPT CQNCFTVKTP LWRRDEHGTV LCNACGLFLK
70 80 90 100 110 120
LHGEPRPISL KTDTIKSRNR KKLNNNNVNT NANTHSNDPN KIFKRKKRLL TTGGGSLPTN
130 140 150 160 170 180
NPKVSILEKF MVSGSIKPLL KPKETVPNTK ECSTQRGKFS LDPCEPSGKN YLYQINGSDI
190 200 210 220 230 240
YTSNIELTRL PNLSTLLEPS PFSDSAVPEI ELTWKLHNEE EVIKLKTKIS ELELVTDLYK
250 260
KHIFQLNEKC KQLEVELHSR ASVQSHPQH
References and
documentation are available.
Number of amino acids: 269
Molecular weight: 30166.45
Theoretical pI: 8.81
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1324
Hydrogen H 2144
Nitrogen N 374
Oxygen O 409
Sulfur S 10
Formula: C
1324H
2144N
374O
409S
10
Total number of atoms: 4261
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17460
Abs 0.1% (=1 g/l) 0.579, assuming all pairs of Cys residues form cystines
Ext. coefficient 16960
Abs 0.1% (=1 g/l) 0.562, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.71
This classifies the protein as stable.
Aliphatic index: 81.86
Grand average of hydropathicity (GRAVY): -0.638