ProtParam
User-provided sequence:
10 20 30 40 50 60
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS
70 80
TILCTPTGGK AKLSEGTSFR RK
References and
documentation are available.
Number of amino acids: 82
Molecular weight: 8879.37
Theoretical pI: 9.36
Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 11
Atomic composition:
Carbon C 387
Hydrogen H 639
Nitrogen N 111
Oxygen O 115
Sulfur S 6
Formula: C
387H
639N
111O
115S
6
Total number of atoms: 1258
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 1740
Abs 0.1% (=1 g/l) 0.196, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.168, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.50
This classifies the protein as unstable.
Aliphatic index: 80.85
Grand average of hydropathicity (GRAVY): -0.082