ProtParam
User-provided sequence:
10 20 30 40 50 60
MVMEERSCHT ANRVGDVQMG NGHKRGRSED SSSNNVSIGV ETDKQDMLSN KAQKKWVFGK
70 80 90 100 110 120
CVNLSAVDAP CFKEMMTVDG GQMGLESSDC HDLNGWRLQD ALEEVQDRVE KIKESWAITG
130 140 150 160 170 180
CSILLDAWVD QKGRDLVTFV ADCPAGLVYL ISFDVSDFKD DVTALLSLVN GLVEEVGVRN
190 200 210 220 230 240
VTQIIACSTS GWVGELGELF AGHDREVFWS VSVSHCFELM LVKISKIRSF GDIFDKVNNI
250 260 270 280 290 300
WLFINNNPSV LNIFRDQCHG IDITVSSSEF EFVTPYLILE SIFKAKKNLT AMFASSNWNN
310 320 330 340 350 360
EQCIAISNLV SDSSFWETVE SVLKCTSPLI HGLLLFSTAN NQHLGYVYDT MDSIKESIAR
370 380 390 400 410 420
EFNHKPQFYK PLWDVIDDVW NKHLHNPLHA AGYFLNPTAF YSTNFHLDIE VVTGLISSLI
430 440 450 460 470 480
HMVEDCHVQF KISTQIDMYR LGKDCFNEAS QADQITGISP AEWWAHKASQ YPELQSLAIK
490 500 510 520 530 540
ILSQTCEGAS KYKLKRSLAE KLLLSEGMSN RERQHLDELV FVQYNLHLQS YKAKLSEEID
VYKD
References and
documentation are available.
Number of amino acids: 544
Molecular weight: 61455.71
Theoretical pI: 5.17
Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2737
Hydrogen H 4234
Nitrogen N 726
Oxygen O 830
Sulfur S 27
Formula: C
2737H
4234N
726O
830S
27
Total number of atoms: 8554
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 91745
Abs 0.1% (=1 g/l) 1.493, assuming all pairs of Cys residues form cystines
Ext. coefficient 90870
Abs 0.1% (=1 g/l) 1.479, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.07
This classifies the protein as stable.
Aliphatic index: 89.91
Grand average of hydropathicity (GRAVY): -0.172