ProtParam
User-provided sequence:
10 20 30 40 50 60
MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ
70 80 90 100 110 120
QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD
130 140 150 160 170 180
IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE
190 200 210 220 230 240
KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN
250 260 270 280 290 300
DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET
310 320 330 340 350 360
NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY
370 380 390 400 410
LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK
References and
documentation are available.
Number of amino acids: 414
Molecular weight: 47437.59
Theoretical pI: 5.63
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2136
Hydrogen H 3248
Nitrogen N 564
Oxygen O 633
Sulfur S 15
Formula: C
2136H
3248N
564O
633S
15
Total number of atoms: 6596
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 86665
Abs 0.1% (=1 g/l) 1.827, assuming all pairs of Cys residues form cystines
Ext. coefficient 86290
Abs 0.1% (=1 g/l) 1.819, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.47
This classifies the protein as stable.
Aliphatic index: 75.12
Grand average of hydropathicity (GRAVY): -0.497