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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVNFDLGQVG EVFRRKDKGA IVSGDNPEEE EDVDASEFEE DEVKPVRTKN RRPKEDAFTQ

70 80 90 100 110 120
QRLAAINPVL TPRTVLPLYL LIAVVFVIVG GCILAQNSKV DEVTIYYQDC MTNATSSWSD

130 140 150 160 170 180
IPSEHWQFVF HKYKTYNTAP QWRFVDDESD DFTKQRGTCQ IRFTTPSDMK NNVYLNYVLE

190 200 210 220 230 240
KFAANHRRYV LSFSEDQIRG EDASYETVHD ATGINCKPLS KNADGKIYYP CGLIANSMFN

250 260 270 280 290 300
DTFPLQLTNV GDTSNNYSLT NKGINWESDK KRYKKTKYNY TQIAPPPYWE KMYPDGYNET

310 320 330 340 350 360
NIPDIQDWEE FQNWMRPGAF DKITKLIRIN KNDTLPAGEY QLDIGLHWPV LEFNGKKGIY

370 380 390 400 410
LTHGSHLGGR NPFLGIVYLI GGCICAAMAL ILLTFWLFGG RKIADASSLS WNMK


References and documentation are available.

Number of amino acids: 414 Molecular weight: 47437.59 Theoretical pI: 5.63
Amino acid composition: 
Ala (A) 23 5.6% Arg (R) 18 4.3% Asn (N) 29 7.0% Asp (D) 29 7.0% Cys (C) 7 1.7% Gln (Q) 15 3.6% Glu (E) 24 5.8% Gly (G) 27 6.5% His (H) 7 1.7% Ile (I) 26 6.3% Leu (L) 30 7.2% Lys (K) 28 6.8% Met (M) 8 1.9% Phe (F) 20 4.8% Pro (P) 21 5.1% Ser (S) 21 5.1% Thr (T) 26 6.3% Trp (W) 10 2.4% Tyr (Y) 21 5.1% Val (V) 24 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 53 Total number of positively charged residues (Arg + Lys): 46 Atomic composition: Carbon C 2136 Hydrogen H 3248 Nitrogen N 564 Oxygen O 633 Sulfur S 15 Formula: C2136H3248N564O633S15 Total number of atoms: 6596 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 86665 Abs 0.1% (=1 g/l) 1.827, assuming all pairs of Cys residues form cystines Ext. coefficient 86290 Abs 0.1% (=1 g/l) 1.819, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 29.47 This classifies the protein as stable. Aliphatic index: 75.12 Grand average of hydropathicity (GRAVY): -0.497