ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVNNSQHPYI KDGWFREIND KSFPGQAFTM TVDSILYEAR SEFQDILIFR NKVYGTVLVL 

        70         80         90        100        110        120 
DGIVQCTEFD EFAYQEMITH IAMFAHSNPK RVLIIGGGDG GVLREVAKHS CVEDITMVEI 

       130        140        150        160        170        180 
DSSVIELSRK FLPTLSNGAF DDERLDLKLC DGFKFLQDIG ASDVHKKFDV IITDSSDPEG 

       190        200        210        220        230        240 
PAEAFFQERY FELLKDALNP NGVVIMQSSE NFWLNLKYLH DLKNTAKKVF PNTEYCYTMV 

       250        260        270        280        290        300 
PTYTSGQLGL IVCSNNANIP LNIPQRKISE QEQGKLKYYN PQIHSSAFVL PTWADKVINE 

References and documentation are available.

Number of amino acids: 300

Molecular weight: 34090.76

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  15	  5.0%
Arg (R)   9	  3.0%
Asn (N)  18	  6.0%
Asp (D)  21	  7.0%
Cys (C)   5	  1.7%
Gln (Q)  13	  4.3%
Glu (E)  20	  6.7%
Gly (G)  17	  5.7%
His (H)   7	  2.3%
Ile (I)  23	  7.7%
Leu (L)  25	  8.3%
Lys (K)  19	  6.3%
Met (M)   7	  2.3%
Phe (F)  19	  6.3%
Pro (P)  13	  4.3%
Ser (S)  20	  6.7%
Thr (T)  14	  4.7%
Trp (W)   3	  1.0%
Tyr (Y)  11	  3.7%
Val (V)  21	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1537
Hydrogen    H	      2364
Nitrogen    N	       394
Oxygen      O	       459
Sulfur      S	        12

Formula: C1537H2364N394O459S12
Total number of atoms: 4766

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33140
Abs 0.1% (=1 g/l)   0.972, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.965, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.28
This classifies the protein as stable.



Aliphatic index: 87.70

Grand average of hydropathicity (GRAVY): -0.222