ProtParam
User-provided sequence:
10 20 30 40 50 60
MVNSHGIRYI RLKQVFNRAL DQSISKLQSW DKVSSCFPQY VNSKQGAINV ANCQRQLTEF
70 80 90 100 110 120
WTELCQREFK EIMEERNVEQ KLNELDELIL EAKERYTDRD QDEVNKGPAI DELSSKELVE
130 140 150 160 170 180
CHLYSQRMHA IHEIDERLAK VNEMNDQLAQ ELKDLETQVE VEKNEIGKMY DEYLGSHTDQ
190 200
PANVLLVQSL NDMVLELKEN Y
References and
documentation are available.
Number of amino acids: 201
Molecular weight: 23639.55
Theoretical pI: 4.78
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1025
Hydrogen H 1631
Nitrogen N 287
Oxygen O 334
Sulfur S 10
Formula: C
1025H
1631N
287O
334S
10
Total number of atoms: 3287
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21680
Abs 0.1% (=1 g/l) 0.917, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.907, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.62
This classifies the protein as unstable.
Aliphatic index: 86.77
Grand average of hydropathicity (GRAVY): -0.755