ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVNTRPPKID ELLKIDPYLH DFQDEISRRY GVFLDYQRRI EECGGMEEFT SSYKQFGLNV 

        70         80         90        100        110        120 
QPDNSVKGLE WAPAAEKLAL IGDFNNWDQN ANVYKKEEHG KWSITVPAKE DGSCPIPHNS 

       130        140        150        160        170        180 
VIKIAVSRHG ATHFKLSPWA TFVTCPNPKE TVIYHQNFWN PPEKYQLKEA RPARPASLRI 

       190        200        210        220        230        240 
YEAHVGISSS EGKINTYREF ADDVLPRIQK QGYNAIQLMA VMEHVYYASF GYQVSNFFAV 

       250        260        270        280        290        300 
SSRCGTPEDL KYLVDKAHSL GIFMLLDVVH SHASKNVEDG LNQWDGSNGG YFHDNARGYH 

       310        320        330        340        350        360 
NLWDSRLFDY TQTETLRFLL SNVRWWVEEY GFDGFRFDGV SSMIYHSHGM NDDFCGGYPM 

       370        380        390        400        410        420 
YFGLNADTDS LVYLMLANDF LHKKYPFMIT IAEEVSGMPG ICRPVEEGGQ GFDYRLAMAL 

       430        440        450        460        470        480 
PDMWIKILKH TSDEDWKIDD IVFNLENRRY AEKHVAYAES HDQALVGDKT IAFWLMDKEM 

       490        500        510        520        530        540 
YDFMSTDSPL TPIIDRGLSL HKLIRLITIG LGGEAWLNFI GNEFGHPEWL DFPRVGNGES 

       550        560        570        580        590        600 
FHYARRQFNL VDAEYLRYKF LNNWDREMML LEERTGFLHK GYAYTSWKHD GDKTIVFERG 

       610        620        630        640        650        660 
GLVFVINLHP TKSFADYSIG VNTPGRYRIA LNSDESKFGG HNRIDNSIKF HTTDDGYAGR 

       670        680 
RHRLQVYITC RTAIVLEKED D 

References and documentation are available.

Number of amino acids: 681

Molecular weight: 78613.56

Theoretical pI: 5.73

Amino acid composition: 
Ala (A)  39	  5.7%
Arg (R)  37	  5.4%
Asn (N)  36	  5.3%
Asp (D)  50	  7.3%
Cys (C)   7	  1.0%
Gln (Q)  17	  2.5%
Glu (E)  44	  6.5%
Gly (G)  52	  7.6%
His (H)  28	  4.1%
Ile (I)  39	  5.7%
Leu (L)  54	  7.9%
Lys (K)  36	  5.3%
Met (M)  18	  2.6%
Phe (F)  39	  5.7%
Pro (P)  28	  4.1%
Ser (S)  40	  5.9%
Thr (T)  28	  4.1%
Trp (W)  16	  2.3%
Tyr (Y)  36	  5.3%
Val (V)  37	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      3553
Hydrogen    H	      5315
Nitrogen    N	       953
Oxygen      O	      1027
Sulfur      S	        25

Formula: C3553H5315N953O1027S25
Total number of atoms: 10873

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   142015
Abs 0.1% (=1 g/l)   1.806, assuming all pairs of Cys residues form cystines


Ext. coefficient   141640
Abs 0.1% (=1 g/l)   1.802, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.29
This classifies the protein as stable.



Aliphatic index: 74.74

Grand average of hydropathicity (GRAVY): -0.473