ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVNWQTLFMV SLRRQGSSSR YRYKFNMENI THQVFPRCKQ AFKKTNLSYE YCDLEGVLYN 

        70         80         90        100        110        120 
ISLTDLQKLL LRDINAPREH AFKIVRTDLT QKSSKKRIQH WERIAPMFDH PLSLYEKLFS 

       130        140        150        160        170        180 
EMDEDFKPSF EWQQLIRVRC KDDKLKLQRV IWPKSIFSNF CRGIGVKKST YDRLLEQNNG 

       190        200        210        220        230        240 
EVPMFVNPAN AKPLPLFQVS DEAMIGEFDG IGIFPYFVDK HREFFVTEVD KLKTKIASPL 

       250        260        270        280        290        300 
CTLNERKRIE KANAGRILAN EEGKPFYLDA NSATTRIAGG NVVTLKQLLE RSVSHKTLWS 

       310        320        330        340        350        360 
KQSNKDRTCP GDILRATILS NDFSIRQLRA EFCKNFILYN IFTILQRNKK SIRSFSGNDN 

       370        380        390        400        410        420 
APSFQFSWNV WDSYIWKQYQ ETESMKIPTD QASLINYKTK YDSFLHDLQT YSALVISEMK 

       430        440        450        460        470        480 
WNQFSIFQND ETTLSRFEHI TLILQTVLTK SKMIRIFQPN LYKFMQDDLR PTLMELVGFT 

       490        500        510        520        530        540 
ESINATIEPG FANEQSLQSA NGLKKLANQL LYFEQEIYGE KFRVNRPIQL RPITLSANYK 

       550        560        570        580        590        600 
IVILDKKNAI PEIFQTLLKF MTQITTYFVK DLSEVELHGH MHCIDKKMLD KSKFMYLYEE 

       610        620        630 
KSNEEVKAAS PQKEKIVDNI IGLLSNDEEH 

References and documentation are available.

Number of amino acids: 630

Molecular weight: 73861.02

Theoretical pI: 9.09

Amino acid composition: 
Ala (A)  27	  4.3%
Arg (R)  33	  5.2%
Asn (N)  38	  6.0%
Asp (D)  32	  5.1%
Cys (C)   8	  1.3%
Gln (Q)  34	  5.4%
Glu (E)  43	  6.8%
Gly (G)  20	  3.2%
His (H)  12	  1.9%
Ile (I)  47	  7.5%
Leu (L)  63	 10.0%
Lys (K)  56	  8.9%
Met (M)  16	  2.5%
Phe (F)  40	  6.3%
Pro (P)  23	  3.7%
Ser (S)  46	  7.3%
Thr (T)  34	  5.4%
Trp (W)   9	  1.4%
Tyr (Y)  22	  3.5%
Val (V)  27	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      3337
Hydrogen    H	      5224
Nitrogen    N	       890
Oxygen      O	       955
Sulfur      S	        24

Formula: C3337H5224N890O955S24
Total number of atoms: 10430

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82780
Abs 0.1% (=1 g/l)   1.121, assuming all pairs of Cys residues form cystines


Ext. coefficient    82280
Abs 0.1% (=1 g/l)   1.114, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.92
This classifies the protein as unstable.



Aliphatic index: 84.81

Grand average of hydropathicity (GRAVY): -0.455