ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVPLEDLLNK ENGTAAPQHS RESIVENGTD VSNVTKKDGL PSPNLSKRSS DCSKRPRIRC 

        70         80         90        100        110        120 
TTEAIGLNGQ EDERMSPGST SSSCLPYHST SHLNTPPYDL LGASAVSPTT SSSSDSSSSS 

       130        140        150        160        170        180 
PLAQAHNPAG DDDDADNDGD SEDITLYCKW DNCGMIFNQP ELLYNHLCHD HVGRKSHKNL 

       190        200        210        220        230        240 
QLNCHWGDCT TKTEKRDHIT SHLRVHVPLK PFGCSTCSKK FKRPQDLKKH LKIHLESGGI 

       250        260        270        280        290        300 
LKRKRGPKWG SKRTSKKNKS CASDAVSSCS ASVPSAIAGS FKSHSTSPQI LPPLPVGISQ 

       310        320        330        340        350        360 
HLPSQQQQRA ISLNQLCSDE LSQYKPVYSP QLSARLQTIL PPLYYNNGST VSQGANSRSM 

       370        380        390        400        410        420 
NVYEDGCSNK TIANATQFFT KLSRNMTNNY ILQQSGGSTE SSSSSGRIPV AQTSYVQPPN 

       430        440        450        460        470        480 
APSYQSVQGG SSISATANTA TYVPVRLAKY PTGPSLTEHL PPLHSNTAGG VFNRQSQYAM 

       490        500        510        520        530        540 
PHYPSVRAAP SYSSSGCSIL PPLQSKIPML PSRRTMAGGT SLKPNWEFSL NQKSCTNDII 

       550        560        570        580        590        600 
MSKLAIEEVD DESEIEDDFV EMLGIVNIIK DYLLCCVMED LDDEESEDKD EENAFLQESL 

       610        620 
EKLSLQNQMG TNSVRILTKY PKILV 

References and documentation are available.

Number of amino acids: 625

Molecular weight: 68232.26

Theoretical pI: 6.83

Amino acid composition: 
Ala (A)  32	  5.1%
Arg (R)  24	  3.8%
Asn (N)  36	  5.8%
Asp (D)  34	  5.4%
Cys (C)  18	  2.9%
Gln (Q)  31	  5.0%
Glu (E)  30	  4.8%
Gly (G)  35	  5.6%
His (H)  19	  3.0%
Ile (I)  28	  4.5%
Leu (L)  55	  8.8%
Lys (K)  38	  6.1%
Met (M)  12	  1.9%
Phe (F)  10	  1.6%
Pro (P)  45	  7.2%
Ser (S)  91	 14.6%
Thr (T)  37	  5.9%
Trp (W)   4	  0.6%
Tyr (Y)  19	  3.0%
Val (V)  27	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2935
Hydrogen    H	      4672
Nitrogen    N	       844
Oxygen      O	       968
Sulfur      S	        30

Formula: C2935H4672N844O968S30
Total number of atoms: 9449

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51435
Abs 0.1% (=1 g/l)   0.754, assuming all pairs of Cys residues form cystines


Ext. coefficient    50310
Abs 0.1% (=1 g/l)   0.737, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 67.57
This classifies the protein as unstable.



Aliphatic index: 69.44

Grand average of hydropathicity (GRAVY): -0.619