ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVPTQVTIFS IIMYVLESLV IIVQSCTTVA VLFREWMHFQ RLSPVETILI SLGISHFCLQ 

        70         80         90        100        110        120 
WTSMLYNFGT YSRPVLLFWK VSVVWEFMNI LTFWLTSWLA VLYCVKVSSF THPIFLWLRM 

       130        140        150        160        170        180 
KILKLVLWLI LGALIASCLS IIPSVVKYHI QMELVTLDNL PKNNSLILRL QQFEWYFSNP 

       190        200        210        220        230        240 
LKMIGFGIPF FVFLASIILL TVSLVQHWVQ MKHYSSSNSS LKAQFTVLKS LATFFTFFTS 

       250        260        270        280        290 
YFLTIVISFI GTVFDKKSWF WVCEAVIYGL VCIHFTSLMM SNPALKKALK LQFWSPEPS 

References and documentation are available.

Number of amino acids: 299

Molecular weight: 34760.77

Theoretical pI: 9.39

Amino acid composition: 
Ala (A)  10	  3.3%
Arg (R)   5	  1.7%
Asn (N)   8	  2.7%
Asp (D)   2	  0.7%
Cys (C)   6	  2.0%
Gln (Q)  11	  3.7%
Glu (E)   8	  2.7%
Gly (G)   7	  2.3%
His (H)   7	  2.3%
Ile (I)  26	  8.7%
Leu (L)  43	 14.4%
Lys (K)  15	  5.0%
Met (M)  11	  3.7%
Phe (F)  27	  9.0%
Pro (P)  11	  3.7%
Ser (S)  32	 10.7%
Thr (T)  19	  6.4%
Trp (W)  13	  4.3%
Tyr (Y)   9	  3.0%
Val (V)  29	  9.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1667
Hydrogen    H	      2537
Nitrogen    N	       375
Oxygen      O	       399
Sulfur      S	        17

Formula: C1667H2537N375O399S17
Total number of atoms: 4995

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    85285
Abs 0.1% (=1 g/l)   2.453, assuming all pairs of Cys residues form cystines


Ext. coefficient    84910
Abs 0.1% (=1 g/l)   2.443, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.82
This classifies the protein as unstable.



Aliphatic index: 121.47

Grand average of hydropathicity (GRAVY): 0.816