ProtParam
User-provided sequence:
10 20 30 40 50 60
MVQAIKLNDL KNRKRKNVEE ENGSDESEID ISSTDSENEE EQNGEEEIVN IDFDFFGGNP
70 80 90 100 110 120
EVDFHALKNL LRQLFGPQES TRIQLSSLAD LILGSPTTTI KTDGKESDPY CFLSFVDFKA
130 140 150 160 170 180
NHLSDYVKYL QKVDMRLSTF FKTMIDSGNK NCALVLSERL INMPPEVVPP LYKITLEDVA
190 200 210 220 230 240
TALGDDKHYD FYIIVTRKYE VNFDTDDDTD SGKRNKNKDE RSKKRVKADE VDYFHEEDRF
250 260 270 280
FEKYAKIHFE SEAKKGVISS YMILDHEGLV KSIDELETEI STW
References and
documentation are available.
Number of amino acids: 283
Molecular weight: 32661.40
Theoretical pI: 4.69
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1445
Hydrogen H 2247
Nitrogen N 377
Oxygen O 471
Sulfur S 7
Formula: C
1445H
2247N
377O
471S
7
Total number of atoms: 4547
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20525
Abs 0.1% (=1 g/l) 0.628, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.625, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.20
This classifies the protein as unstable.
Aliphatic index: 77.46
Grand average of hydropathicity (GRAVY): -0.693