ProtParam
User-provided sequence:
10 20 30 40 50 60
MVRATVLNVG DHASEGVRTN KAKGEKMVLE PPMNSAQRRK LGDITNLQNQ KNLMNQGAKH
70 80 90 100 110 120
QQQAILISSK ENAENLQKAL RNSSENTKLM KVVMERDGIK SDLKKLRIEF QKVQEQNLLL
130 140 150 160 170 180
AQANTRILAL KVLQHELGCK NGLVMARKML LKAQANACGG ACKTFQPNDA DHEHASGSSN
190 200 210 220 230 240
ANSLQRNEKA NSKRRVSGRK NPANSEVLDI IGRSGETCQM EDNIDNKKLV SDSDNDAENH
250 260 270 280 290 300
INDNVQSKRY CAGRQSSSSK TREASQTETL QKVVDAKEIK GDARFSLTKH SDWLKSQEPE
310 320 330 340 350 360
PSESLYESRF PLRRRSARLK SQEPEPSESF HDSIETTKRR RSAIRSAMFN IQELGVIQNL
370 380 390 400 410 420
NGLPDDQEIA AKARCSAREQ STGSKPEAVE PHDTKEIIGK SRISLRRQSA RFNFQELGVT
430 440 450 460 470 480
ENLNGPHDDQ TIAANARCCA SEQSIGSKPE AVEPHDIEER IGKIRVSSRR QSANIETPRA
490 500 510 520 530 540
IKEPANPPLH DDNVEESSQI SCSVSMELKR ESKKKPTGDE SEEMRKTTVG RPSRQAAEKI
550
KSYKEPSLKE KMRGGF
References and
documentation are available.
Number of amino acids: 556
Molecular weight: 62044.62
Theoretical pI: 9.31
Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 94
Atomic composition:
Carbon C 2614
Hydrogen H 4341
Nitrogen N 835
Oxygen O 867
Sulfur S 22
Formula: C
2614H
4341N
835O
867S
22
Total number of atoms: 8679
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10470
Abs 0.1% (=1 g/l) 0.169, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.161, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 64.35
This classifies the protein as unstable.
Aliphatic index: 67.61
Grand average of hydropathicity (GRAVY): -0.972