ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVRFGDELGG RYGGTGGGER ARGGGAGGAG GPGQGGLPPG QRVLYKQSIA QRARTMALYN 

        70         80         90        100        110        120 
PIPVKQNCFT VNRSLFVFSE DNVVRKYAKR ITEWPPFEYM ILATIIANCI VLALEQHLPD 

       130        140        150        160        170        180 
GDKTPMSERL DDTEPYFIGI FCFEAGIKII ALGFVFHKGS YLRNGWNVMD FVVVLTGILA 

       190        200        210        220        230        240 
TAGTDFDLRT LRAVRVLRPL KLVSGIPSLQ VVLKSIMKAM VPLLQIGLLL FFAILMFAII 

       250        260        270        280        290        300 
GLEFYMGKFH KACFPNSTDA EPVGDFPCGK EAPARLCDSD TECREYWPGP NFGITNFDNI 

       310        320        330        340        350        360 
LFAILTVFQC ITMEGWTDIL YNTNDAAGNT WNWLYFIPLI IIGSFFMLNL VLGVLSGEFA 

       370        380        390        400        410        420 
KERERVENRR AFLKLRRQQQ IERELNGYLE WIFKAEEVML AEEDKNAEEK SPLDVLKRAA 

       430        440        450        460        470        480 
TKKSRNDLIH AEEGEDRFVD LCAAGSPFAR ASLKSGKTES SSYFRRKEKM FRFLIRRMVK 

       490        500        510        520        530        540 
AQSFYWVVLC VVALNTLCVA MVHYNQPQRL TTALYFAEFV FLGLFLTEMS LKMYGLGPRS 

       550        560        570        580        590        600 
YFRSSFNCFD FGVIVGSIFE VVWAAIKPGT SFGISVLRAL RLLRIFKVTK YWNSLRNLVV 

       610        620        630        640        650        660 
SLLNSMKSII SLLFLLFLFI VVFALLGMQL FGGQFNFQDE TPTTNFDTFP AAILTVFQIL 

       670        680        690        700        710        720 
TGEDWNAVMY HGIESQGGVS KGMFSSFYFI VLTLFGNYTL LNVFLAIAVD NLANAQELTK 

       730        740        750        760        770        780 
DEEEMEEAAN QKLALQKAKE VAEVSPMSAA NISIAARQQN SAKARSVWEQ RASQLRLQNL 

       790        800        810        820        830        840 
RASCEALYSE MDPEERLRYA STRHVRPDMK THMDRPLVVE PGRDGLRGPA GNKSKPEGTE 

       850        860        870        880        890        900 
ATEGADPPRR HHRHRDRDKT SASTPAGGEQ DRTDCPKAES TETGAREERA RPRRSHSKEA 

       910        920        930        940        950        960 
PGADTQVRCE RSRRHHRRGS PEEATEREPR RHRAHRHAQD SSKEGKEGTA PVLVPKGERR 

       970        980        990       1000       1010       1020 
ARHRGPRTGP RETENSEEPT RRHRAKHKVP PTLEPPEREV AEKESNVVEG DKETRNHQPK 

      1030       1040       1050       1060       1070       1080 
EPRCDLEAIA VTGVGSLHML PSTCLQKVDE QPEDADNQRN VTRMGSQPSD PSTTVHVPVT 

      1090       1100       1110       1120       1130       1140 
LTGPPGEATV VPSANTDLEG QAEGKKEAEA DDVLRRGPRP IVPYSSMFCL SPTNLLRRFC 

      1150       1160       1170       1180       1190       1200 
HYIVTMRYFE MVILVVIALS SIALAAEDPV RTDSFRNNAL KYMDYIFTGV FTFEMVIKMI 

      1210       1220       1230       1240       1250       1260 
DLGLLLHPGA YFRDLWNILD FIVVSGALVA FAFSGSKGKD INTIKSLRVL RVLRPLKTIK 

      1270       1280       1290       1300       1310       1320 
RLPKLKAVFD CVVNSLKNVL NILIVYMLFM FIFAVIAVQL FKGKFFYCTD ESKELERDCR 

      1330       1340       1350       1360       1370       1380 
GQYLDYEKEE VEAQPRQWKK YDFHYDNVLW ALLTLFTVST GEGWPMVLKH SVDATYEEQG 

      1390       1400       1410       1420       1430       1440 
PSPGFRMELS IFYVVYFVVF PFFFVNIFVA LIIITFQEQG DKVMSECSLE KNERACIDFA 

      1450       1460       1470       1480       1490       1500 
ISAKPLTRYM PQNKQSFQYK TWTFVVSPPF EYFIMAMIAL NTVVLMMKFY DAPYEYELML 

      1510       1520       1530       1540       1550       1560 
KCLNIVFTSM FSLECILKII AFGVLNYFRD AWNVFDFVTV LGSITDILVT EIAETNNFIN 

      1570       1580       1590       1600       1610       1620 
LSFLRLFRAA RLIKLLRQGY TIRILLWTFV QSFKALPYVC LLIAMLFFIY AIIGMQVFGN 

      1630       1640       1650       1660       1670       1680 
IALDDGTSIN RHNNFRTFLQ ALMLLFRSAT GEAWHEIMLS CLGNRACDPH ANASECGSDF 

      1690       1700       1710       1720       1730       1740 
AYFYFVSFIF LCSFLMLNLF VAVIMDNFEY LTRDSSILGP HHLDEFIRVW AEYDPAACGR 

      1750       1760       1770       1780       1790       1800 
ISYNDMFEML KHMSPPLGLG KKCPARVAYK RLVRMNMPIS NEDMTVHFTS TLMALIRTAL 

      1810       1820       1830       1840       1850       1860 
EIKLAPAGTK QHQCDAELRK EISSVWANLP QKTLDLLVPP HKPDEMTVGK VYAALMIFDF 

      1870       1880       1890       1900       1910       1920 
YKQNKTTRDQ THQAPGGLSQ MGPVSLFHPL KATLEQTQPA VLRGARVFLR QKSATSLSNG 

      1930       1940       1950       1960       1970       1980 
GAIQTQESGI KESLSWGTQR TQDVLYEARA PLERGHSAEI PVGQPGALAV DVQMQNMTLR 

      1990       2000       2010       2020       2030       2040 
GPDGEPQPGL ESQGRAASMP RLAAETQPAP NASPMKRSIS TLAPRPHGTQ LCNTVLDRPP 

      2050       2060       2070       2080       2090       2100 
PSQVSHHHHH RCHRRRDKKQ RSLEKGPSLS VDTEGAPSTA AGSGLPHGEG STGCRRERKQ 

      2110       2120       2130       2140       2150       2160 
ERGRSQERRQ PSSSSSEKQR FYSCDRFGSR EPPQPKPSLS SHPISPTAAL EPGPHPQGSG 

      2170       2180       2190       2200       2210       2220 
SVNGSPLMST SGASTPGRGG RRQLPQTPLT PRPSITYKTA NSSPVHFAEG QSGLPAFSPG 

      2230       2240       2250       2260       2270       2280 
RLSRGLSEHN ALLQKEPLSQ PLASGSRIGS DPYLGQRLDS EASAHNLPED TLTFEEAVAT 

      2290       2300       2310       2320       2330 
NSGRSSRTSY VSSLTSQSHP LRRVPNGYHC TLGLSTGVRA RHSYHHPDQD HWC 

References and documentation are available.

Number of amino acids: 2333

Molecular weight: 261930.76

Theoretical pI: 8.83

Amino acid composition: 
Ala (A) 179	  7.7%
Arg (R) 168	  7.2%
Asn (N)  86	  3.7%
Asp (D)  94	  4.0%
Cys (C)  40	  1.7%
Gln (Q)  88	  3.8%
Glu (E) 151	  6.5%
Gly (G) 157	  6.7%
His (H)  58	  2.5%
Ile (I) 109	  4.7%
Leu (L) 228	  9.8%
Lys (K) 105	  4.5%
Met (M)  67	  2.9%
Phe (F) 133	  5.7%
Pro (P) 140	  6.0%
Ser (S) 168	  7.2%
Thr (T) 128	  5.5%
Trp (W)  24	  1.0%
Tyr (Y)  64	  2.7%
Val (V) 146	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 245
Total number of positively charged residues (Arg + Lys): 273

Atomic composition:

Carbon      C	     11712
Hydrogen    H	     18350
Nitrogen    N	      3256
Oxygen      O	      3358
Sulfur      S	       107

Formula: C11712H18350N3256O3358S107
Total number of atoms: 36783

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   229860
Abs 0.1% (=1 g/l)   0.878, assuming all pairs of Cys residues form cystines


Ext. coefficient   227360
Abs 0.1% (=1 g/l)   0.868, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.65
This classifies the protein as unstable.



Aliphatic index: 82.16

Grand average of hydropathicity (GRAVY): -0.232