ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVRFVSILSL FGCAATLVTA HDDMDMDMDM DMDMDMNIDT TTSQSIDVSS TASIVPVPHE 

        70         80         90        100        110        120 
PKHLHGLPIL QSPSLTPAER LYWENYNTTT YFTTQAGNRS ALRYHIITLL LVAFVLYPVS 

       130        140        150        160        170        180 
LALSAARSRW YLPLLFVNLC ICISSVMALS VFKNTFPEED WYAHNIYGTT SVLLLVFMLV 

       190        200        210        220        230        240 
HFFAAVLSVP VSLASKKEYR PVDTIPLNDL ESTPVMVNSA RGSPSPSSNR DTLFSLSSDT 

       250        260        270        280        290        300 
TTATATNNNK RRRAEGEDEG DNTSNHDTLR DEDYDNDDDE IASIEAPPLL PQDIPVFRIL 

       310        320        330        340        350        360 
FTNTKYQMLA AHLSCVANVV FHMLTYPLFM YIFVDLIIGF AVGNLLGKGI RIFNLLAHWI 

       370        380        390        400        410        420 
KGGVFFTLGV VSLARYCGFA AKYGWAWNNI SFTSQLTQTR SSNLLFRFAP AGTFTMEFVE 

       430        440        450        460        470        480 
SFLIFFYGST NIFLEHLAGN GGAWTAKDLQ HVSIAFMFIG TGLCGLLTEY KLNHWRFEHA 

       490        500        510        520        530        540 
RKRPQTDVVA ATPGYSPNPF PAFTIFWTGI LMSQHAQSSQ FSTTIHTQWG YLLSYGSFFR 

       550        560        570        580        590        600 
LLTFLILFLV PNTNSAASKP FTELITSFCL LCGGLVFMES TDQSIEAMEY RGFTPMFTFN 

       610        620        630 
LSVGFVSLLM AWEMILFIWK DWLIKTRKTS L 

References and documentation are available.

Number of amino acids: 631

Molecular weight: 70921.59

Theoretical pI: 5.70

Amino acid composition: 
Ala (A)  46	  7.3%
Arg (R)  24	  3.8%
Asn (N)  30	  4.8%
Asp (D)  30	  4.8%
Cys (C)   8	  1.3%
Gln (Q)  14	  2.2%
Glu (E)  23	  3.6%
Gly (G)  32	  5.1%
His (H)  17	  2.7%
Ile (I)  35	  5.5%
Leu (L)  74	 11.7%
Lys (K)  16	  2.5%
Met (M)  22	  3.5%
Phe (F)  49	  7.8%
Pro (P)  29	  4.6%
Ser (S)  55	  8.7%
Thr (T)  55	  8.7%
Trp (W)  13	  2.1%
Tyr (Y)  21	  3.3%
Val (V)  38	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      3250
Hydrogen    H	      4932
Nitrogen    N	       810
Oxygen      O	       913
Sulfur      S	        30

Formula: C3250H4932N810O913S30
Total number of atoms: 9935

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   103290
Abs 0.1% (=1 g/l)   1.456, assuming all pairs of Cys residues form cystines


Ext. coefficient   102790
Abs 0.1% (=1 g/l)   1.449, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.56
This classifies the protein as unstable.



Aliphatic index: 92.12

Grand average of hydropathicity (GRAVY): 0.214