ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE 

        70         80         90        100        110        120 
EEVLAMDEDD ESIDEREDEE EEEEEELDGA AAYKKIFGRN LETDQLPEED EENGMLDNEN 

       130        140        150        160        170        180 
AWGSTKGEYY GADDLDDDEA AKEIEKEALR QQKKHLEELN MNDYLDEEEE EEWVKSAKEF 

       190        200        210        220        230        240 
DMGEFKNSTK QADTKTSITD ILNMDDEARD NYLRTMFPEF APLSKEFTEL APKFDELKKS 

       250        260        270        280        290        300 
EENEFNKLKL IALGSYLGTI SCYYSILLHE LHNNEDFTSM KGHPVMEKIL TTKEIWRQAS 

       310        320        330        340        350        360 
ELPSSFDVNE GDGSESEETA NIEAFNEKKL NELQNSEDSD AEDGGKQKQE IDEEERESDE 

       370        380        390        400        410        420 
EEEEEDVDID DFEEYVAQSR LHSKPKTSSM PEADDFIESE IADVDAQDKK ARRRTLRFYT 

       430        440        450        460        470        480 
SKIDQQENKK TDRFKGDDDI PYKERLFERQ QRLLDEARKR GMHDNNGADL DDKDYGSEDE 

       490        500        510        520        530        540 
AVSRSINTQG ENDYYQQVQR GKQDKKISRK EAHKNAVIAA REGKLAELAE NVSGDGKRAI 

       550        560        570        580        590        600 
NYQILKNKGL TPKRNKDNRN SRVKKRKKYQ KAQKKLKSVR AVYSGGQSGV YEGEKTGIKK 

       610 
GLTRSVKFKN 

References and documentation are available.

Number of amino acids: 610

Molecular weight: 70259.40

Theoretical pI: 4.59

Amino acid composition: 
Ala (A)  37	  6.1%
Arg (R)  32	  5.2%
Asn (N)  35	  5.7%
Asp (D)  65	 10.7%
Cys (C)   1	  0.2%
Gln (Q)  24	  3.9%
Glu (E)  93	 15.2%
Gly (G)  35	  5.7%
His (H)   8	  1.3%
Ile (I)  24	  3.9%
Leu (L)  43	  7.0%
Lys (K)  65	 10.7%
Met (M)  11	  1.8%
Phe (F)  19	  3.1%
Pro (P)  11	  1.8%
Ser (S)  41	  6.7%
Thr (T)  24	  3.9%
Trp (W)   3	  0.5%
Tyr (Y)  19	  3.1%
Val (V)  20	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 158
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3005
Hydrogen    H	      4733
Nitrogen    N	       849
Oxygen      O	      1070
Sulfur      S	        12

Formula: C3005H4733N849O1070S12
Total number of atoms: 9669

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44810
Abs 0.1% (=1 g/l)   0.638, assuming all pairs of Cys residues form cystines


Ext. coefficient    44810
Abs 0.1% (=1 g/l)   0.638, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.93
This classifies the protein as unstable.



Aliphatic index: 58.41

Grand average of hydropathicity (GRAVY): -1.299