ProtParam
User-provided sequence:
10 20 30 40 50 60
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST HGRYAGEVSH
70 80 90 100 110 120
DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF KELDTAQKHI DAGAKKVVIT
130 140 150 160 170 180
APSSTAPMFV MGVNEEKYTS DLKIVSNASC TTNCLAPLAK VINDAFGIEE GLMTTVHSMT
190 200 210 220 230 240
ATQKTVDGPS HKDWRGGRTA SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV
250 260 270 280 290 300
VDLTVKLNKE TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ
310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA
References and
documentation are available.
Number of amino acids: 332
Molecular weight: 35846.85
Theoretical pI: 6.46
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1592
Hydrogen H 2538
Nitrogen N 430
Oxygen O 489
Sulfur S 10
Formula: C
1592H
2538N
430O
489S
10
Total number of atoms: 5059
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31525
Abs 0.1% (=1 g/l) 0.879, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.876, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.95
This classifies the protein as stable.
Aliphatic index: 89.55
Grand average of hydropathicity (GRAVY): -0.093