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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST HGRYAGEVSH

70 80 90 100 110 120
DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF KELDTAQKHI DAGAKKVVIT

130 140 150 160 170 180
APSSTAPMFV MGVNEEKYTS DLKIVSNASC TTNCLAPLAK VINDAFGIEE GLMTTVHSMT

190 200 210 220 230 240
ATQKTVDGPS HKDWRGGRTA SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV

250 260 270 280 290 300
VDLTVKLNKE TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ

310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA


References and documentation are available.

Number of amino acids: 332 Molecular weight: 35846.85 Theoretical pI: 6.46
Amino acid composition: 
Ala (A) 33 9.9% Arg (R) 11 3.3% Asn (N) 14 4.2% Asp (D) 24 7.2% Cys (C) 2 0.6% Gln (Q) 6 1.8% Glu (E) 15 4.5% Gly (G) 25 7.5% His (H) 8 2.4% Ile (I) 20 6.0% Leu (L) 21 6.3% Lys (K) 26 7.8% Met (M) 8 2.4% Phe (F) 11 3.3% Pro (P) 11 3.3% Ser (S) 25 7.5% Thr (T) 23 6.9% Trp (W) 3 0.9% Tyr (Y) 10 3.0% Val (V) 36 10.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 37 Atomic composition: Carbon C 1592 Hydrogen H 2538 Nitrogen N 430 Oxygen O 489 Sulfur S 10 Formula: C1592H2538N430O489S10 Total number of atoms: 5059 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 31525 Abs 0.1% (=1 g/l) 0.879, assuming all pairs of Cys residues form cystines Ext. coefficient 31400 Abs 0.1% (=1 g/l) 0.876, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.95 This classifies the protein as stable. Aliphatic index: 89.55 Grand average of hydropathicity (GRAVY): -0.093