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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVRVAINGFG RIGRLVMRIA LSRPNVEVVA LNDPFITNDY AAYMFKYDST HGRYAGEVSH

70 80 90 100 110 120
DDKHIIVDGK KIATYQERDP ANLPWGSSNV DIAIDSTGVF KELDTAQKHI DAGAKKVVIT

130 140 150 160 170 180
APSSTAPMFV MGVNEEKYTS DLKIVSNASC TTNCLAPLAK VINDAFGIEE GLMTTVHSLT

190 200 210 220 230 240
ATQKTVDGPS HKDWRGGRTA SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV

250 260 270 280 290 300
VDLTVKLNKE TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSHS SIFDASAGIQ

310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA


References and documentation are available.

Number of amino acids: 332 Molecular weight: 35746.67 Theoretical pI: 6.46
Amino acid composition: 
Ala (A) 32 9.6% Arg (R) 11 3.3% Asn (N) 13 3.9% Asp (D) 24 7.2% Cys (C) 2 0.6% Gln (Q) 5 1.5% Glu (E) 15 4.5% Gly (G) 26 7.8% His (H) 8 2.4% Ile (I) 19 5.7% Leu (L) 21 6.3% Lys (K) 26 7.8% Met (M) 7 2.1% Phe (F) 10 3.0% Pro (P) 12 3.6% Ser (S) 26 7.8% Thr (T) 24 7.2% Trp (W) 3 0.9% Tyr (Y) 11 3.3% Val (V) 37 11.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 37 Atomic composition: Carbon C 1588 Hydrogen H 2530 Nitrogen N 428 Oxygen O 490 Sulfur S 9 Formula: C1588H2530N428O490S9 Total number of atoms: 5045 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 33015 Abs 0.1% (=1 g/l) 0.924, assuming all pairs of Cys residues form cystines Ext. coefficient 32890 Abs 0.1% (=1 g/l) 0.920, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 27.07 This classifies the protein as stable. Aliphatic index: 88.95 Grand average of hydropathicity (GRAVY): -0.107