ProtParam
User-provided sequence:
10 20 30 40 50 60
MVRVEKIKTK KGKRVLVNRA SKTVENDKKA LFCRGAKTNE IISHAMMDLF DMKKPLTTKM
70 80 90 100 110 120
DKHNPYHLFE DETPIVRAGS KFDTSLFVLG SNSKKKPNCL TFGRTYDGQL LDMAELRITS
130 140 150 160 170 180
YKSSSNFEAA KMTLGSKPCV ILEGAAFESD GDMKRIGNLM VDWFRGPKVD TVRLEGLETV
190 200 210 220 230 240
IVFTALDETN LALRVYRPML KKSATATPRV ELAEMGPSIS FEVMRKKLAD DALFKLACKK
250 260 270 280 290
PKALMKKRRK NLSEDVFGNQ LARVHVGKQR TDDIQTRKVK ALRKTPLVEA APENAIE
References and
documentation are available.
Number of amino acids: 297
Molecular weight: 33460.18
Theoretical pI: 9.78
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 1477
Hydrogen H 2439
Nitrogen N 419
Oxygen O 428
Sulfur S 17
Formula: C
1477H
2439N
419O
428S
17
Total number of atoms: 4780
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11710
Abs 0.1% (=1 g/l) 0.350, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.342, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.94
This classifies the protein as stable.
Aliphatic index: 81.11
Grand average of hydropathicity (GRAVY): -0.433