ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVSEDKIEQW KATKVIGIIG LGDMGLLYAN KFTDAGWGVI CCDREEYYDE LKEKYASAKF 

        70         80         90        100        110        120 
ELVKNGHLVS RQSDYIIYSV EASNISKIVA TYGPSSKVGT IVGGQTSCKL PEIEAFEKYL 

       130        140        150        160        170        180 
PKDCDIITVH SLHGPKVNTE GQPLVIINHR SQYPESFEFV NSVMACLKSK QVYLTYEEHD 

       190        200        210        220        230        240 
KITADTQAVT HAAFLSMGSA WAKIKIYPWT LGVNKWYGGL ENVKVNISLR IYSNKWHVYA 

       250        260        270        280        290        300 
GLAITNPSAH QQILQYATSA TELFSLMIDN KEQELTDRLL KAKQFVFGKH TGLLLLDDTI 

       310        320        330        340        350        360 
LEKYSLSKSS IGNSNNCKPV PNSHLSLLAI VDSWFQLGID PYDHMICSTP LFRIFLGVSE 

       370        380        390        400        410        420 
YLFLKPGLLE QTIDAAIHDK SFIKDDLEFV ISAREWSSVV SFANFDIYKK QFQSVQKFFE 

       430        440        450 
PMLPEANLIG NEMIKTILSH SSDRSAAEKR NT 

References and documentation are available.

Number of amino acids: 452

Molecular weight: 50923.33

Theoretical pI: 6.13

Amino acid composition: 
Ala (A)  29	  6.4%
Arg (R)   9	  2.0%
Asn (N)  20	  4.4%
Asp (D)  23	  5.1%
Cys (C)   7	  1.5%
Gln (Q)  17	  3.8%
Glu (E)  29	  6.4%
Gly (G)  25	  5.5%
His (H)  13	  2.9%
Ile (I)  37	  8.2%
Leu (L)  43	  9.5%
Lys (K)  37	  8.2%
Met (M)   8	  1.8%
Phe (F)  20	  4.4%
Pro (P)  15	  3.3%
Ser (S)  42	  9.3%
Thr (T)  22	  4.9%
Trp (W)   8	  1.8%
Tyr (Y)  20	  4.4%
Val (V)  28	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2311
Hydrogen    H	      3587
Nitrogen    N	       587
Oxygen      O	       678
Sulfur      S	        15

Formula: C2311H3587N587O678S15
Total number of atoms: 7178

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74175
Abs 0.1% (=1 g/l)   1.457, assuming all pairs of Cys residues form cystines


Ext. coefficient    73800
Abs 0.1% (=1 g/l)   1.449, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.96
This classifies the protein as stable.



Aliphatic index: 93.41

Grand average of hydropathicity (GRAVY): -0.145