ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVSFLSSSVS RLPLRIAGRR IPGRFAVPQV RTYAKDLKFG VDARASLLTG VDTLARAVSV 

        70         80         90        100        110        120 
TLGPKGRNVL IDQPFGSPKI TKDGVTVARS VSLKDKFENL GARLVQDVAS KTNEVAGDGT 

       130        140        150        160        170        180 
TTATVLTRAI FSETVRNVAA GCNPMDLRRG IQLAVDNVVE FLQANKRDIT TSEEISQVAT 

       190        200        210        220        230        240 
ISANGDTHIG ELLAKAMERV GKEGVITVKE GRTISDELEV TEGMKFDRGY ISPYFITDVK 

       250        260        270        280        290        300 
SQKVEFENPL ILLSEKKVSA VQDILPSLEL AAQQRRPLVI IAEDVDGEAL AACILNKLRG 

       310        320        330        340        350        360 
QLQVVAIKAP GFGDNRRNML GDLAVLTDSA VFNDEIDVSI EKAQPHHLGS CGSVTVTKED 

       370        380        390        400        410        420 
TIIMKGAGDH VKVNDRCEQI RGVMADPNLT EYEKEKLQER LAKLSGGIAV IKVGGSSEVE 

       430        440        450        460        470        480 
VNEKKDRIVD ALNAVKAAVS EGVLPGAGTS FVKASLRLGD IPTNNFDQKL GVEIVRKAIT 

       490        500        510        520        530        540 
RPAQTILENA GLEGNLIVGK LKELYGKEFN IGYDIAKDRF VDLNEIGVLD PLKVVRTGLV 

       550        560        570        580 
DASGVASLMG TTECAIVDAP EESKAPAGPP GMGGMGGMPG MM 

References and documentation are available.

Number of amino acids: 582

Molecular weight: 62167.42

Theoretical pI: 5.76

Amino acid composition: 
Ala (A)  53	  9.1%
Arg (R)  33	  5.7%
Asn (N)  23	  4.0%
Asp (D)  37	  6.4%
Cys (C)   5	  0.9%
Gln (Q)  17	  2.9%
Glu (E)  40	  6.9%
Gly (G)  55	  9.5%
His (H)   4	  0.7%
Ile (I)  37	  6.4%
Leu (L)  53	  9.1%
Lys (K)  39	  6.7%
Met (M)  13	  2.2%
Phe (F)  16	  2.7%
Pro (P)  23	  4.0%
Ser (S)  34	  5.8%
Thr (T)  33	  5.7%
Trp (W)   0	  0.0%
Tyr (Y)   6	  1.0%
Val (V)  61	 10.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2722
Hydrogen    H	      4504
Nitrogen    N	       768
Oxygen      O	       850
Sulfur      S	        18

Formula: C2722H4504N768O850S18
Total number of atoms: 8862

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9190
Abs 0.1% (=1 g/l)   0.148, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.144, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.22
This classifies the protein as stable.



Aliphatic index: 99.81

Grand average of hydropathicity (GRAVY): -0.066